Research on macrophage secretion regulation ingredients in Bidens genus plants

我们检测了小花鬼针草对巨噬细胞分泌NO的抑制作用和前列腺素E_2(PGE_2)对巨噬细胞分泌的抑制作用，这与抗过敏和慢性炎症有关。中药鬼针草为菊科植物，是一种传统的解热、抗风湿药物。用脂多糖和干扰素-γ激活小鼠巨噬细胞样细胞系RAW264.7，检测了61个化合物对一氧化氮(NO)产生和大鼠腹膜肥大细胞释放组胺的抗过敏作用。我们分离了五个新的聚乙炔糖苷、二十五个含有两个新化合物的黄酮类化合物、一个新的二聚咖啡酸衍生物、两个新的咖啡酸酯、两个咖啡酸衍生物、两个肉桂酸衍生物、两个新的新木脂素、一个具有十个碳链的环戊烯糖苷、八个奎宁酸酯、一个苯醇糖苷、一个萘吡酮糖苷、胡萝卜素、二萜、甾醇和肌醇。在五个聚乙酰乙烯糖中，有四个对NO的产生有很强的抑制活性，并且，关于对这些AMGAMGAMAGE的无产品性能的影响，在用干扰素-γ和脂多糖复活的药物试验中，发现抑制被认为是药物作用的结果。在黄酮类化合物中，3‘，4’-位含两个羟基的黄酮类、黄酮醇类和月桂酮类化合物不能识别产物抑制活性。此外，咖啡酸二聚体(一种甲基奎宁酸的咖啡酸酯)没有识别出任何产物抑制活性。我们研究了分离的化合物对内毒素激活的巨噬细胞产生前列腺素(PG)E_2的抑制作用。对于蔗糖对羟基肉桂酸酯和咖啡酸衍生物，PGE_2的生成抑制活性被几种化合物所识别。

We examined Bidens parviflora with NO production inhibitory effects and prostaglandin E_2 (PGE_2) inhibitory effect toward the macrophage secretions which had a relation in antiallergy and chronic inflammation. Chinese folk medicine, Bidens parviflora (Compositae) has been used as a traditional antipyretic and anti-rheumatic medicine. Sixty-one compounds isolated from this whole plant were examined for anti-allergic actively toward nitric oxide (NO) production by a murine macrophage-like cell line, RAW264.7, activated with lipopolysaccharide (LPS) and interferon-γ(IFN-γ) and histamine release from rat peritoneal mast cells in vitro. We isolated five new polyacetylene glucosides, twenty-five flavonoides containing two new compounds, a new dimeric caffeic acid derivative, two new caffeic acid esters, two caffeic acid derivatives, two cinnamic acid derivatives, two new neolignans, a cyclopentene glycoside having ten carbon chain, eight quinic acid esters, a benzyl alcohol glycoside, a naphthopyrone glycoside, carotinoids, a diterpene, sterols and myo-inositol.Among five polyacetilene glucosides, four exhibited strong inhibitory activities toward NO production and, about effect to give to NO product performance of a macrophage of these amalgam, restraint was recognized as a result of having examined action of an examination drug at having been revitalized with IFN-γ and LPS. In the flavonoids, NO product inhibitory activities were recognized by flavones, flavonols and aurones which had two hydroxyl groups to 3', 4' -position. In addition, NO product inhibitory activities were recognized, by a caffeic acid dimer, a caffeic acid ester of methyl quinic acid. We investigated the inhibitory effects of isolated compounds on prostaglandin (PG) E_2 production in LPS-activated macrophages. About sucrose p-hydroxylcinnamic acid esters and caffeic acid derivatives, PGE_2 production inhibitory activities were recognized by several compounds.