Optical mapping of neuronal activities in the submucous plexus with single cell resolution

为了确定豚鼠粘膜下神经丛神经网络的功能单位，采用电压敏感的苯乙烯染料（di-8-ANEPPS）和464元件光电二极管阵列，采用单细胞分辨率的跨膜电压活动多点光学记录（MSORTV）来监测神经元的自发电活动，神经丛中的分离神经节由这些神经元组成。原始光学数据的尖峰分选分析用于识别活动神经元并分离其单独的尖峰序列。计算机生成的动画用于显示神经元活动的模式。从不同神经节中的神经元记录的准偶发性爆发的初步观察表明存在神经节上神经元网络，其形成粘膜下神经丛的功能单位。

In order to identify functional units of the neural network within the submucous plexus of the guinea pig, multiple-site optical recording of transmembrane voltage activity (MSORTV) with single cell resolution, using a voltage-sensitive styryl dye (di-8-ANEPPS) and a 464 element photodiode array, were employed to monitor the spontaneous electrical activity of the neurons of which the separate ganglia in the plexus are composed. Spike sorting analysis of the raw optical data is used to identify the active neurons and isolate their individual spike trains. Computer generated animations are used to show the patterns of neuronal activity. Preliminary observations of quasicoincident bursts recorded from neurons in separate ganglia suggest the existence of supraganglionic neuronal networks, which form the functional units of the submucous plexus.