Establishment of Genomics Based Hepatology

根据本研究的四个目的，对研究结果进行了总结：1.肝病基因表达谱数据库的构建，结合临床资料，构建了世界上最大的人类肝脏疾病基因表达谱数据库; 2.肝病分析，综合分析慢性B肝炎、丙型肝炎、自身免疫性肝炎、乙型肝炎、丙型肝炎、乙型肝炎利用SAGE技术和DNA芯片技术对原发性胆汁性肝硬化、肝细胞癌进行了基因表达系列分析，并在这些疾病的诊断和发病机制的理解方面取得了新的发现。3.开发了分析、诊断和治疗肝脏疾病的新方法。这种方法能够发现染色体区域中的组织特异性基因。通过基因表达谱的综合分析，成功地预测了肝病治疗的疗效。4.肝脏异常的检测，这是不被认为是重要的疾病。糖尿病肝脏中的基因表达与非糖尿病肝脏中的基因表达明显不同。此外，与血管生成相关的基因在肝脏中表达上调，表明糖尿病肝脏与包括动脉粥样硬化在内的全身性并发症有直接关系。

Results are summarized according to the four objects of this study.1.Construction of comprehensive gene expression profile database of liver diseases.Data base of gene expression profile of liver diseases were constructed with the clinical information, which is the biggest human liver database in the world.2.Analysis of liver diseases.Comprehensive analysis of gene expression of samples from chronic hepatitis B and C, autoimmune hepatitis, primary biliary cirrhosis, hepatocellular carcinoma were performed using serial analysis of gene expression (SAGE) and DNA chips, and demonstrated new findings on the diagnosis and understanding of the pathogenesis of those diseases.3.Development of new methods for the analysis, diagnosis, and treatment of liver diseases.Tissue specific region was found in the human chromosome by using the database #1. This method enables the discovery of tissue specific gene in the chromosomal region. The efficacy of treatment for liver disease was successfully predicted by the comprehensive gene expression analysis.4.Detection of hepatic abnormality, which is not considered as important diseases.Expression profile of diabetic liver with or without obesity was comprehensively analyzed. Gene expression in the diabetic liver was clearly different from that in non-diabetic liver. Furthermore, genes relating with angiogenesis were up-regulating in the liver, indicating that diabetic liver is directly relating with systemic complication including atherosclerosis.