The control of bacterial infectious diseases by the hamony of immune system and nerve system

探讨了儿茶酚胺受体在宿主抵抗细菌感染中的作用机制。此外，还研究了巨噬细胞移动抑制因子(MIF)在细菌感染中的作用，MIF被认为是介导免疫系统和神经系统之间的串扰。儿茶酚胺在宿主防御细菌感染中的调节机制。研究了肾上腺素受体拮抗剂和激动剂对宿主抵抗单核细胞增多性李斯特菌感染和细胞因子应答的影响。小鼠注射肾上腺素受体α拮抗剂后，对李斯特菌的耐药性和干扰素-γ、肿瘤坏死因子-α的产生均有抑制作用。相反，表肾上腺素受体β拮抗剂对李斯特菌耐药性和细胞因子的产生没有显著影响。这些结果提示，γ和α的产生可能受肾上腺素受体α的调节。微血管生成因子在细菌…中的作用更多的感染(1)单核细胞增多性李氏杆菌致死性感染可上调感染器官内源性MIF水平。给小鼠注射抗MIF抗体对单核细胞增多性李斯特氏菌亚致死性感染的命运没有显著影响，但抗体将小鼠从致死性单核细胞增多性李斯特菌感染中拯救出来。(2)在致死性感染的早期，内源性干扰素-γ和肿瘤坏死因子-α的产生在致死性感染小鼠体内表达上调，而在致死性感染的晚期表达下调。(3)抗MIF抗体处理组小鼠肝脏内源性IL-10生成上调。给予抗MIF抗体处理的小鼠，给予抗IL-10抗体可部分恢复肝功能的异常。(4)抗MIF抗体处理组小鼠血浆皮质醇水平显著低于正常兔球蛋白处理组小鼠，提示MIF通过调节IL-10的产生参与了致死性单核细胞增多性李斯特菌感染的发病过程。较少

The host defense mechanism against bacterial infections through catecholamine receptors was investigated. Furthermore, the role of macrophage migration inhibitory factor (MIF), which is known to mediate the crosstalk between immune system and nerve system, in bacterial infections was investigated.1. The regulatory mechanism by catecholamines in host defense against bacterial infections. The in vivo effects of antagonists and agonists to epinephrine receptors on host defense against Listeria monocytgenes infection and cytokine responses were investigated. When epinephrine receptor α antagonists were injected into mice, antilisterial resistance and productions of IFN-γ and TNF-α were suppressed. In contrast, epimephrine receptor β antagonists revealed no significant effect on antilisterial resistance and the cytokine production. These results suggest that antilisterial resistance and productions of IFN-γ and TNF-α may be regulated by epinephrine receptor α.2. The role of MIF in bacterial … More infections (1) Endogenous MIF levels in the infected organs were up-regulated by a lethal infection with L. monocytogenes. The administration of anti-MIF antibody into mice showed no significant effect on the fate of a sublethal infection with L. monocytogenes, whereas the antibody rescued the mice from a lethal infection with L. monocytogenes. (2) Endogenous IFN-γ and TNF-α production in the infected organs was up-regulated in the early phase but down-regulated in the late phase of lethal infection in anti-MIF antibody-treated mice. (3) Endogenous IL-10 production in the livers was up-regulated in anti-MIF antibody-treated mice. Abnormality of liver function was partially restored by the administration of anti-IL-10 antibody in anti-MIF antibody-treated mice. (4) The level of plasma cortisol was significantly low in anti-MIF antibody-treated mice, compared with that of the normal rabbit globulin-treated mice.These results suggest that MIF is involved in the pathogensis of a lethal infection with L. monocytogenes through the regulation of IL-10 production. Less