STUDY ON PATHOPHYSIOLOGICAL SIGNIFICANCIE OF BILIRUBIN

背景：随着活性氧的病理生理意义被发现，围产期胆红素的抗氧化作用也逐渐被发现。胆红素清除或猝灭活性氧物种和过氧化脂质等。清除的胆红素被氧化成支持物，猝灭的胆红素被氧化成胆绿素。然而，这些胆红素氧化产物在新生儿期的病理生理意义尚不清楚。方法在体外实验中，我们测定了黄嘌呤-黄嘌呤氧化酶体系在O_2~-作用下形成的胆红素氧化产物的量，无论是作为游离胆红素还是作为胆红素-人血清白蛋白络合物。在体内研究中，我们测量了光疗或氧疗产生的血清胆绿素和尿液中胆绿素的量。分别用Biopyrrin EIA试剂盒和8-OHdG-EIA试剂盒检测新生儿黄褐斑光疗前后尿液中的生物吡啶和8-羟基脱氧鸟苷。结果：在体外实验中，两种反应液中均有明显的支持物生成，但未检测到胆绿素。在体内研究中，胆绿素没有反映在活性氧物种的产生上。由于胆红素的光异构体，Biopyrrin很难评价胆红素的抗氧化作用，而8-羟基脱氧鸟苷由于新生儿尿液中的杂质，也很难评价其产生的活性氧物种。结论：(ZZ)-胆红素在体内生产支持物还需要进一步的研究

Background : The antioxidant effect of bilirubin in perinatal period came to be found as the pathophysiological significance of active oxygen was found. Bilirubin scavenges or quenches active oxygen species and lipid peroxides etc. Scavenged bilirubin is oxidized to propentdyopents, and quenched bilirubin is oxidized to biliverdin. However, the pathophysiological significance of these bilirubin oxidative products in neonatal period is unclear. We studied the bilirubin oxidative productsMethods In an In vitro study we measured the amounts of oxidative products of bilirubin formed through the action of O_2^-by the xanthine-xanthine oxidase system, either as free bilirubin or as bilirubin-human serum albumin complex. In an in vivo study we measured the amounts of serum biliverdin and urinary propentdyopents that were produced by phototherapy or oxygen therapy. We measured biopyrrin examined by using a Biopyrrin EIA kit and 8-hydroxydeoxyguanosine examined by using an 8-OhdG ELISA kit in neonatal urine before and after neonatal jaundiced phototherapyResults : In the in vitro study, a significant formation of propentdyopents was observed in the both reaction solution, but biliverdin was not detected. In the in vivo study, biliverdin was not reflected in generation of active oxygen species. Biopyrrin was difficult to evaluate 'the antioxidant effects of bilirubin because of bilirubin photoisomers and also 8-hydroxydeoxyguanosine was difficult to evaluate the production of active oxygen species because of the impurities in neonatal urine. Conclusion : Further study on the production of propentdyopents production from (ZZ)-bilirubin in vivo is needed