The Lole of Local Regulator in the Rat Ovary during Ovulatory Process

白细胞介素8（IL-8）家族中的一种白细胞介素诱导的嗜中性粒细胞趋化因子（CINC/gro）是大鼠中性粒细胞的功能性趋化因子。在本研究中，我们研究了CINC/gro是否有助于大鼠排卵系统中的排卵过程。在大鼠卵巢中，CINC/gro在窦卵泡的卵泡膜层中被化学识别，但在颗粒细胞中不被识别。为了阐明CINC/gro在排卵过程中的作用，在孕马血清促性腺激素（PMSG）-hCG处理期间检测了CINC/gro蛋白和mRNA。注射PMSG后CINC/gro蛋白没有增加。注射hCG后，CINC/gro mRNA表达迅速增加，6 h达高峰。CINC/gro蛋白表达随IL-1 β和肿瘤坏死因子（TNF）的升高而升高。在全卵巢分散培养中，FSH、hCG、IL 1 β和TNF以剂量依赖方式刺激CINC/gro蛋白的产生。尤其是IL 1β和TNF的刺激作用强于促性腺激素。腹腔注射抗CINC/gro抗体中和内源性CINC/gro效应的组输卵管冲洗液中显微镜下计数的排卵卵母细胞数显著低于对照组。这些结果表明，CINC/gro通过吸引中性粒细胞在大鼠排卵过程中起着重要作用。CINC/gro在排卵前升高，可能受IL-1 Q、TNF等细胞因子的直接调节，受促性腺激素的间接调节

A cytokine-induced neutriophil chemoattractant (CINC/gro), which belongs to the interieukin (IL)-8 family, acts as a functional chemoattractant for neutrophils in rats. In the present study, we examined whether CINC/gro contributes to the ovulation process in the rat ovulation system. In rat ovaries, CINC/gro was immunohistochemicaliy recognized in the theca layer of the antral follicle but not in the granulosa cells. To clarify the role of CINC/gro in the ovulation process, CINC/gro protein and mRNA were examined during pregnant mare serum gonadotropin (PMSG)-hCG treatment. CINC/gro protein did not increase as a result of PMSG injection. However, it increased rapidly after hCG injection and peaked at 6 h after hCG.CINC/gro mRNA was also strongly expressed after hCG injection. The increase of CINC/gro protein followed increases in IL 1 p and tumor necrosis factor (TNF). In the whole ovarian dispersate culture, FSH, hCG, IL 1 p, and TNF stimulated the production of CINC/gro protein in a dose-dependent manner. In particular, the stimulatory effects of IL 1β and TNF were stronger than those of gonadotropins. The number of ovulated ooCytes counted under microscopy in the salpinx wash solution was significantly lower in the group which endogenous CINC/gro effect was neutralized by intraperitoneal injection of the antibody to CINC/gro than in the control group. These results suggest that CINC/gro plays an important role in the rat ovulation process by attracting neutrophils. CINC/gro increased just prior to ovulation, and it may be regulated directly by cytokines such as IL 1 Q and TNF and indirectly by gonadotropins