Expression of heat shock proteins in the monkey eyes

1.热休克蛋白在灵长类青光眼视网膜中的表达检测眼压和视神经头，用免疫组织化学方法检测抗HSP抗体。神经保护性HSP(90、60、27)在青光眼视网膜较正常视网膜表达上调。HSP70在青光眼视网膜中也呈中度升高。参与细胞外物质重塑的HSP47在青光眼视网膜中的表达没有改变。用Zeiss DAB分析系统对这种免疫反应进行了定量，除HSP47.2外，其余HSP分子均有明显变化。热休克蛋白在灵长类动物实验性青光眼视神经头中的表达除HSP-47外，所有HSP在视神经头中的表达均降低。免疫组织化学显示HSP47和HSP70在筛板表达增强。原位杂交显示HSP70和HSP47在筛板表达增加。与前板层和后板层相比，筛板的HSP表达增加可能在该特定区域的重塑中起重要作用，被认为在青光眼神经病变中起重要作用。在青光眼中，HSP在视网膜和视神经头中的表达不同，HSP的作用有待于进一步研究，以期控制HSP的表达成为青光眼神经病变的神经保护措施。

1. Expression of Heat Shock Protein in the primate glaucomatous retinaExperimental glaucoma model was made by repeated exposure of argon laser to the chamber angle. Intraocular pressure and optic nerve head was then monitored.Immunohistochemistry was performed for panels of antibodies against HSPs.Neuroprotective HSP (90,60,27) were upregulated in the glaucomatous retina compared with that of control normal retina. HSP 70 was also moderately elevated in the glaucomatous retina. Expression of HSP 47, act to extracellular material remodeling was not altered in glaucomatous retina. Such immunoreactivity was quantificated by Zeiss DAB analysis system and significantly change was obtained for all HSP molecules except that of HSP 47.2. Expression of Heat Shock Protein in the optic nerve heads of primate experimental glaucoma.Expression of HSP was decreased in the optic nerve head in all HSP except for HSP 47. In lamina cribrosa, HSP 47 and 70 showed enhanced expression by immunohistochemistry. In situ hybridization also demonstrated increased expression of HSP 70 and 47 at lamina cribrosa. Lamina cribrosa, contrast to prelamina and retro laminal portion, showed increased expression of HSP may indicate important role for the remodeling of this specific area, which is believed to play an important role for glaucomatous neuropathy.As a summary, HSP may play an important role for the pathogenesis of glaucomatous neuropathy. Expression of HSP was different between retina and optic nerve head in glaucomatous eyes.Further study should be warranted to clear the role of HSP for future possibility whether controlling the HSP expression become neuroprotective regimen for glaucomatous neuropathy.