Risk assessment of peri-inplantitis patients with chair-side MMP-8 assay system

基质金属蛋白酶（MMPs）在牙周组织的病理性破坏中起重要作用。因此，与骨基质降解相关的MMP可能是种植体周围持续性骨破坏的可能标志物。在这项研究中，四名种植体周围炎患者（男性/女性：2/2 ;平均年龄：68.5^^+__-6.7岁; 9例植入）和年龄和性别匹配的4例对照患者（男/女：2/2，平均年龄：66.0^^+__-5.8岁，8个种植体），从我院1990年至2000年安装种植支持上部结构的64例患者中选择。本研究中选择种植体周围炎患者和对照患者的入选标准分别为上部结构安装后种植体“每年累积垂直骨丢失（ABL）超过0.6 mm”和“每年ABL小于0.2 mm”。种植体周围的骨水平通过每年拍摄的牙科X线片进行评估， ...更多信息 使用三个校准的观察者读数的ES作为结果。还计算了上部结构安装后每年持续的垂直骨丢失（OBL）。用吸水纸尖在龈沟近中颊角收集龈沟液（GCF）。然后将样品溶于含吐温20的0.1%磷酸盐缓冲液（PBS-T）中，并在-20℃下储存。使用对MMP-1、-8和-13具有特异性的抗人单克隆抗体（富士化学工业，日本）和阳性对照，通过蛋白质印迹法检测GCF中的MMP。结果，在种植体周围炎患者中，MMP-8在GCF取样的OBL大于0.8mm的三个活跃骨吸收种植体中被检测到，而在GCF取样的OBL小于0.3mm的其他六个种植体中未检测到，而在对照中未检测到MMP-8。MMP-1和-13在本研究中的任何GCF中均未检测到。这些结果表明，MMP-8可能是预测种植体周围炎种植体周围持续骨破坏的标志物。少

Matrix metalloproteinases (MMPs) are known to play an important role in pathological tissue destruction in the periodontal tissues. Therefore, MMPs related to bone matrix degradation could be a possible marker for ongoing bone destruction around the peri-implantitis implants. In this study, four peri-implantitis patients (male/female : 2/2 ; mean age : 68.5^^+__-6.7 yrs ; 9 implants ) and age- and sex- matched four control patients (male/female : 2/2, mean age : 66.0^^+__-5.8 yrs, 8 implants) were selected from 64 consecutive patients who were installed implant-supported superstructures in our school from 1990 to 2000. Inclusion criteria for selection of the peri-implantitis and control patients in this study were having an implant "with accumulated vertical bone loss (ABL) of more than 0.6 mm per year" and "with ABL of less than 0.2 mm per year" after superstructure installation, respectively. Bone levels around implants were assessed by dental x-ray films taken annually and mean valu … More es of three calibrated observers readings were used as outcome. Ongoing vertical bone loss per year (OBL) after superstructure installation was also calculated. Gingival crevicular fluids (GCFs) were collected at the mesio-buccal comer of the gingival crevices by using absorbent paper points. Then the samples were dissolved in 0.1% phoshate buffered saline with Tween 20 (PBS-T) and stored at -20℃. MMPs in GCFs were detected by Western blotting using anti-human monoclonal antibodies specific for MMP-1, -8 and -13 (Fuji Chemical Industry, Japan) with positive controls. As the results, in the peri-implantitis patients, MMP-8 was detected in three actively bone-resorbing implants whose OBL at GCF sampling was more than 0.8 mm and not detected in the other six implants whose OBL at GCF sampling was less than 0.3 mm, while no MMP-8 was detected in the controls. MMP-1 and -13 could not be detected in any GCFs in this study. These results suggest that MMP-8 might be a marker for prediction of ongoing bone destruction around the peri-implantitis implants. Less