Involvement of immune system to the inferior alveolar nerve injury.

Eliav等人（Pain 83 162-182, 1999）报道大鼠坐骨神经周围神经炎引起痛觉过敏和异位性痛。我们修改了Eliav模型以防止对坐骨神经的物理刺激。oxcel直接放置在坐骨神经上，不需要接触任何器械，并用完全弗氏佐剂（CFA）饱和。术后第1天免疫组化检测ED-1（巨噬细胞标志物）、TNFalpha和il -1 β免疫反应性。TNFalpha和il -1 β免疫反应细胞被认为是雪旺细胞。坐骨神经上髓鞘碱性蛋白消失。这表明，脱乳发生在坐骨神经。此外，背根神经节神经元表达神经肽Y、GAP-43和ATF3，这是已知的在axonized DRG神经元上表达的。提示外周免疫系统的激活可能引起外周神经损伤。

Eliav et al. (Pain 83 162-182, 1999) reported that peripheral neuritis of the rat sciatic nerve induced hyperalgesia and allodynia.We modified the Eliav's model in order to prevent physical stimulation to the sciatic nerve. Oxycel was directly placed on the sciatic nerve without any touch of instruments, and saturated with Complete Freund's Adjuvant (CFA). ED-1 (a marker for macrophages), TNFalpha and IL-1beta immunoreactivity were detected from post-operative day 1 by immunohistochemistry. TNFalpha and IL-1beta immunoreactive cells were thought to be Schwann cells. Myelin basic protein was abolished on the sciatic nerve. This suggests that demuelination was occurred in the sciatic nerve. Furthermore, dorsal root ganglion neurons expressed Neuropeptide Y, GAP-43 and ATF3, which were well known to be expressed on axotomized DRG neurons.Those findings suggested that activating of peripheral immune system might induce peripheral nerve injury.