Development and application of immobilized receptor with the object of screening of drugs activator.

近年来，我们报道了鸡α_1-酸性糖蛋白（chicken α_1-AGP）的氨基酸序列（biochemistry . biophysal . rs . communi .295(2002)587-590）。在这项研究中，我们利用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）鉴定了鸡α_1-AGP的二硫键和位点特异性糖基化，包括氨基甲酰化、氨基甲酰化、完全去糖化的鸡α_1-AGP（cd-α_1-AGP）和未还原的变性cd-α_1-AGP。根据m/z 3037.4（氨基酸序列69 ~ 76 + 161 ~ 183）和3453.3（氨基酸序列69 ~ 80 + 161 ~ 183）处检测到的肽的存在，确定鸡α_1-AGP的两个二硫键的位置为Cys 6-Cys 146和Cys 73-Cys 163。此外，发现Asn 16、70、77和87完全糖基化，Asn 62部分糖基化。

Recently, we reported the amino acid sequence of chicken α_1-acid glycoprotein(chicken α_1-AGP)(Biochem.Biophys.Res.Commun.295(2002)587-590). In this study, we identify disulfide bonds and site-specific glycosylation in chicken α_1-AGP using tryptic digests of carbamidomethylated, chicken α_1-AGP, carbamidomethylated, completely deglycosylated chicken α_1-AGP(cd-α_1-AGP), and non-reduced, denatured cd-α_1-AGP by matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF-MS). Based on the existence of the peptides detected at m/z 3037.4(amino acid sequence 69-76 plus 161-183) and 3453.3(amino acid sequence 69-80 plus 161-183), the location of two disulfide bonds of chicken α_1-AGP was determined to be Cys 6-Cys 146 and Cys 73-Cys 163. Furthermore, it was found that Asn 16,70,77 and 87 were fully glycosylated, and that Asn 62 was partially glycosylated.