The qualitative and quantitative analysis of olfactory glomerulus and nidus in the olfactory bulb

本文对7种哺乳动物的主要嗅球（MOBs）进行了免疫组化研究，重点研究了两点：1)我们之前在实验室麝鼠mob中报道的含有许多GABAergic calbindin D28K-immunoreactive （CB-IR）元素的特殊球形突触区域nidi是否也存在于其他动物中；2)我们在大鼠mob中提出的肾小球的室室组织和两种类型的肾小球周围细胞是否在这些动物的mob中普遍存在。我们使用了两种食虫动物，鼹鼠和刺猬，树鼩，蝙蝠和老鼠，以及实验室麝鼠和老鼠。我们仅在鼹鼠和实验室鼩中发现了离散的中性神经和中性神经层，其中没有观察到从肾小球中挤出的嗅神经（Ons）。这些结果表明，nidi和相关的独特组织可能仅为某些食虫动物所特有。另一方面，我们在所有实验的哺乳动物的mob中都发现了肾小球的明显区室组织和两种类型的肾小球周围细胞，这表明这些结构特征在各种哺乳动物的mob中是共同的，因此可能是mob的重要组织原则。

We investigated immunohistochemically the main olfactory bulbs (MOBs) of 7 mammalian species, focusing on two points: 1) whether nidi, particular spheroidal synaptic regions containing many GABAergic calbindin D28K-immunoreactive (CB-IR) elements we previously reported in the laboratory musk shrew MOBs, are also present in other animals, and 2) whether the compartmental organization of glomeruli and two types of periglomerular cells we proposed in the rat MOBs, are general in the MOBs, of these animals. We used two insectivores, moles and hedgehogs, tree shrews, bats and mice as well as laboratory musk shrews and rats. We found discrete nidi and the nidal layer only in the mole and laboratory shrew MOBs, where observed none or a few olfactory nerves (Ons) extruding from glomeruli. These results suggest that the nidi and related distinctive organization might be specific to only some species of insectivores.On the other hand, we recognized the apparent compartmental organization of glomeruli and two types of periglomerular cells in the MOBs of all of the mammals we examined, indicating these structural features are common to the MOBs of various mammals and thus might be important organizational principles of the MOBs.