The analysis of neuronal mechanism of the nucleus in the midbrain related to calling behavior with sexual difference in birds

本研究的目的是阐明性成熟的孟加拉雀（Lonchura striata vara . domestica）的鸣叫系统中背内侧核（DM）网络在细胞水平上的神经功能和解剖结构，以及在本能行为中产生不同性别的距离鸣叫（DC）的声学结构。远距离呼叫的声学结构表现出性别差异。这种行为的两分法是由于强健的男性纹状体核（RA）发育到性成熟时，从RA到DM的神经输入的神经支配。以上结果是对性成熟男性双侧类风湿性关节炎电解损伤前后远距离呼叫的声学结构分析结果。在双侧RA损伤后，这些雄性发出的叫声在声学上与雌性相似。我们在细胞水平上研究了用细胞膜片钳对强健型纹状核（RA）神经元脉冲的更多排序。本研究采用膜片钳对不同发育阶段雄鸟雄性特异性发声的运动控制核——RA的切片制备神经元进行全细胞记录。我们重点研究了RA神经元神经活动的放电模式，并重点研究了“感觉运动学习”阶段和“结晶后”阶段神经活动的差异。根据电压钳记录下RA神经元的电生理特性，将其分为三种类型。这三种类型分别是“非尖峰”、“单尖峰”和“多尖峰”型神经元。三种类型的分布比例在发展过程中发生了变化。我们比较了这三种神经元在两个不同学习阶段的分布。在感觉运动学习阶段，多尖峰型神经元的分布比例显著高于单尖峰型神经元的分布比例，在结晶后阶段，分布比例之间的关系发生逆转。非尖峰型神经元在两个学习阶段的分布比例无显著差异

The purpose of this study is to clarify the neural function and anatomical structure at the cellular level in network of the dorsomedial nucleus(DM) in the vocal control system and in the producing of sexually different acoustic structures of distance calls(DC) of instinct behavior in sexually mature Bengalese finches (Lonchura striata var. domestica) Immature males and females have acoustically similar distance calls and in sexually mature male, the acoustic structures of distance calls show sexual differences. This behavioral dichotomy is due to innervation of the neural input from RA to DM when robust nucleus of the arichistriatum (RA) in the male developed sexually mature. The above findings are results of analyzing acoustic structures of distance calls before and after electrolytic lesions of the bilateral RA in sexually mature males. After bilateral RA lesioning, these males have produced distance calls acoustically similar to those of female. We studied at the cellular level rec … More ording of the impulses of neurons in the robust nucleus of the archistriatum (RA) by cell patch clamp.In this study, whole cell patch clamp recordings were made from neurons of the slice preparation in the RA, which is a motor control nucleus of male-specific vocalization in male birds of different developmental stage. We focused on the firing patterns in the neural activity of RA neurons, and also focused on the difference in neural activity between the "sensory motor learning" stage and the "after crystallization" stage. The neurons in the RA were categorized into three types according to their electrophysiological properties under voltage clamp recording. The three types are "non-spike", "single-spike" and "multi-spike" type neurons. Distribution ratios of the three types changed during developments. We compared the distribution of the three types of these neurons in the two different learning stages. The distribution ratios of multi-spike type neuron was significantly higher than that of the single-spike type neuron in the sensory motor learning stage, and the relationship between the distribution ratios was reversed in the after crystallization stage. No significant difference between the distribution ratios of the non-spike type neuron in the two learning stages was observed Less