Research for clinical application of gene therapy for head and neck cancer

目的：目的：研究考克斯-2启动子调控的条件复制选择性腺病毒载体（Ad-COX-2-Ela）对头颈部鳞癌细胞的杀伤活性。其溶瘤活性根据考克斯-2和CAR（科萨基和腺病毒受体）的表达水平进行了测试，在一系列的人头颈部鳞状细胞癌细胞line.ResultsThe相应的考克斯-2 mRNA表达率KB，H891，T891，T892，和L 871分别为1.5，60，1，14.6，和1.3。相应的CAR mRNA表达比率为1、1、5、4和3。体外试验显示，考克斯-2强表达的癌细胞系的生长受到显著抑制。此外，Ad-COX 2-Ela对表达考克斯-2的肿瘤有明显的生长抑制作用，提示利用基于考克斯-2启动子的条件复制选择性腺病毒治疗表达考克斯-2的头颈部鳞状细胞癌是可行的。

Objective : To teat the oncolytic activity of COX2-promoter based conditional replication-selective adenovirus vector for squamous cell carcinoma cells of head and neck.Subjects : A conditional replication-selective adenovirus vector in which the expression of Ela, required for viral replication, is controlled by the COX-2 promoter, Ad-COX2-Ela, was generated. Its oncolytic activity according to the levels of COX-2 and CAR (Coxsackie and adenovirus receptor) expression was tested in a series of human head and neck squamous cell carcinoma cell lines.ResultsThe respective COX-2 mRNA expression ratios of KB, H891, T891, T892, and L871 were 1.5, 60, 1, 14.6, and 1.3. The corresponding CAR mRNA expression ratios were 1, 1, 5, 4, and 3. In vitro assays showed significant growth suppression of cancer cell lines with strong expressions of COX-2. In addition, Ad-COX2-Ela showed significant growth suppression of COX-2 expressing tumors in vivo model.CenclusionsIn this study, we demonstrated the possibility of oncolytic therapy using the COX-2 promoter based conditional replication-selective adenovirus for head dnd neck squamous cell carcinoma expressing COX-2.