Brain regional distributions of some transient metals and their bioinorganic chemical studies by the use of multielemental analysis.

为了更好地了解一些瞬态金属的脑区域生物行为及其生物无机化学研究，采用多示踪核探针技术进行了研究。发现了以下内容。1)。采用电感耦合等离子体质谱法（ICP-MS）测定正常Wistar大鼠脑区Mn、Fe、Cu和Zn的浓度，并绘制其分布图，直观比较生长过程各阶段元素的分布。在有关其大脑分布的新发现中，特别值得注意的是，与邻近区域相比，在黑质中观察到高浓度的Mn， Fe和Zn。2)采用上述方法，观察6-羟多巴胺（OHDA）诱导的帕金森病大鼠模型脑内Mn、Fe、Cu和Zn浓度的变化。结果表明，6-羟多巴胺损伤大鼠黑质、杏仁核和壳核中Mn、Fe、Cu和Zn水平升高。模型大鼠黑质中铁含量明显升高。在6-OHDA脑组织中，尤其是黑质中，锰含量的增加比其他元素的增加更早，达到平台期。这四种元素的模式可能是帕金森病发展的关键。3)我们观察到放射性^<86>Rb^+和^<201>Tl^+经小鼠鼻内给药后经嗅神经直接转运。这些结果表明，嗅觉神经通路的轴突运输，从鼻腔到嗅球，再通过突触连接到嗅皮层。^<86>Rb^+和^<201>Tl^+的嗅觉运输应该代表了K^+在嗅觉系统中的行为。

This study was undertaken in order to acquire better understanding of the brain regional biobehavior of some transient metals and their bioinorganic chemical studies by the nuclear probe techniques of the multitracer. The followings were found. 1).Mn, Fe, Cu and Zn concentrations were determined in the brain regions of normal Wistar rats using inductively coupled plasma mass spectrometry (ICP-MS), and their maps were illustrated to compare visually the distribution of the elements at the stage of the growth process. Among new findings about their brain distribution, it is especially worth noting that high concentrations of Mn, Fe and Zn were observed in the substantia nigra compared with those in neighboring regions. 2)Using the above technique, the changes in Mn, Fe, Cu and Zn concentrations were also examined in the brain of a 6-hydroxydopamine (OHDA)-induced rat model of Parkinson's disease. The results indicated that Mn, Fe, Cu and Zn levels were increased in the 6-OHDA-lesioned substantia nigra, amygdala and putamen. A marked increase in Fe level was observed in the substantia nigra of model rats. The increase in manganese level occurred earlier than those in the other elements reaching a plateau in the 6-OHDA brain, especially in the substantia nigra. The patterns in these 4 elements may hold the key to the development of Parkinson's disease. 3)We observed the direct transport of radioactive ^<86>Rb^+ and ^<201>Tl^+ via the olfactory nerve transport following intranasal administration in mice. These results evidently indicated the axonal transport of the via the olfactory nerve pathway, from nasal cavity to the olfactory bulb, and moreover to the olfactory cortex through the synaptic junctions. The olfactory transport of the ^<86>Rb^+ and ^<201>Tl^+ should represent the behavior of K^+ in the olfactory system.