RNA splicing: factors and mechanisms

大多数初级基因转录本必须将其非编码内含子序列拼接出来，然后才能翻译mRNA。此外，选择性剪接使细胞能够产生比细胞核中的基因多得多的蛋白质。基于我们在ZNF265上已被证明的成功，我们将分离新的RNA相互作用因子及其合作伙伴，将它们共同定位在核内室，并阐明它们对前mRNA剪接的影响。这将为人类基因组计划和EST序列信息提供及时的副产品，而在这些信息中，发现只有大约。我们基因组中的30,000个基因突显了选择性剪接在细胞大型蛋白质组库生成中的重要作用。这将给科学、社会和生物技术产业带来可观的好处。

Most primary gene transcripts must have their noncoding intronic sequences spliced out before the mRNA can be translated. Moreover, alternative splicing enables cells to generate a far more proteins than there are genes in the nucleus. Based on our proven success with ZNF265 we will isolate novel RNA interactors and their partners, colocalize these in intranuclear compartments, and elucidate their effect on pre-mRNA splicing. This will provide timely spin-offs to the Human genome Project and EST sequence information, where the finding of only approx. 30,000 genes in our genome highlights the important role of alternative splicing in generating the large proteome repertoire of cells. This will bring considerable benefits to science, society, and the biotech industry.