Development of tissue-engineered cell sheet transplantation for corneal diseases using temperature responsive culture dishes

我们研究了使用温度响应培养皿的组织工程细胞片移植治疗角膜疾病。在这个项目中，我们获得了以下结果。关于角膜上皮细胞片移植的开发项目，（1）我们开发了在温度响应培养皿上培养角膜上皮干细胞的技术，制造多层角膜上皮细胞片并收获它们，（2）我们从角膜缘上皮纯化了SP细胞，并通过使用细胞表面标记的细胞分选富集了角膜上皮祖细胞，（3）我们已经开发了细胞片移植的外科技术。（4）我们已经开发了一种有效的将基因转移到角膜上皮细胞片的方法。关于角膜内皮细胞片移植的开发项目，（5）开发了人角膜内皮细胞的培养方法，（6）开发了在温度响应培养皿上培养角膜内皮细胞、制作角膜内皮细胞片并收获它们的技术。这些结果使我们能够将我们开发的技术用于临床。

We investigated a tissue-engineered cell sheet transplantation for corneal diseases using temperature responsive culture dishes. In this project, we obtained the following results. Regarding the project for development of corneal epithelial cell sheet transplantation, (1) we have developed a technique to culture corneal epithelial stem cells on temperature-responsive culture dishes, fabricate multilayered corneal epithelial cell sheets and harvest them, (2) we have purified SP cells from limbal epithelium and enriched corneal epithelial progenitor cells by cell sorting using cell surface markers, (3) we have developed surgical techniques for transplantation of the cell sheets, (4) we have developed an effective method for gene transfer to the corneal epithelial cell sheets. Regarding the project for development of corneal endothelial cell sheet transplantation, (5) we have developed culture methods of human corneal endothelial cells, (6) we have developed a technique to culture corneal endothelial cells on temperature-responsive culture dishes, fabricate corneal endothelial cell sheets and harvest them. These results allow us to use our developed technologies for clinical use.