Elucidation of interactions between beneficial microorganisms and plants and its mechanism(s) involved in defense system in plant

本研究研究了AMF Glomus mosseae与PGPF Phoma sp.菌株GS8-2、GS8-3、单纯青霉GP17-2和哈茨木霉GT3-2的定殖、促进植物生长和抑制病害的相互作用。哈氏t菌GT3-2对黄瓜根中莫塞菌的定殖有促进作用，而GS8-2、GS8-3和单纯P. GP17-2对黄瓜根中莫塞菌的定殖没有影响。不同菌种加在一起对PGPF建立的影响不同。虽然苔藓藓菌对Phoma sp.菌株的定殖有较强的抑制作用，但对哈茨芽孢杆菌的定殖影响较小。相比之下，G mosseae没有改变单叶茅根和根际定殖。苔藓藓菌也会对Phoma sp.菌株促进生长和诱导全身抗性的能力产生不利影响，而单纯p.s simplicissimum或harzianum则不会对其产生相同的有益影响。相反，T. harzianum促进植物生长的能力被G. mosseae的存在协同增强。单根假单胞菌和哈兹兰假单胞菌与莫塞菌联合接种对根源病原菌茄枯病（R. solani）的抑制效果较好，但对叶片病原菌圆形假单胞菌（C. orbiculare）的抑制效果不明显。

This study investigated the interactions between AMF Glomus mosseae and PGPF Phoma sp. isolates GS8-2, GS8-3, Penicillium simplicissimum GP17-2 or Trichoderma harzianum GT3-2 for their colonization, plant growth promotion and disease suppression. Mycorrhizal colonization by G. mosseae in cucumber roots was enhanced by T. harzianum GT3-2, while Phoma sp. GS8-2, GS8-3 and P. simplicissimum GP17-2 had no effect. The effects of G. mosseae on the establishment of PGPF differ with the different PGPF species combined. Although, the colonization of Phoma sp. isolates was strongly suppressed by G. mosseae, T. harzianum was slightly affected. In contrast, neither the root nor rhizosphere colonization of P. simplicissimum was altered by G mosseae. The ability of Phoma sp. isolates to promote growth and induce systemic resistance was also adversely affected by G. mosseae, however, the same beneficial effects of P. simplicissimum or T. harzianum was not affected. Instead, the ability of T. harzianum to promote plant growth was synergistically enhanced by the presence of G. mosseae. Combined inoculation of P. simplicissimum or T. harzianum with G mosseae resulted in better suppression of the root pathogen R. solani, though such an effect was not observed on the leaf pathogen C. orbiculare.