Development of artificial arrayed nose system with drug metabolizing enzymes

研究了一种新型的具有药物代谢系统的人工阵列鼻系统，包括以下步骤：(A)乙醇蒸气的乙醇脱氢酶荧光光纤；(B)以黄素单加氧酶为药物代谢酶之一的生化嗅探器；(C)丝网印刷电极的生物嗅探芯片。具体研究结果如下：(1)利用依赖NAD+的脱氢酶，以UV-LED和便携式光谱仪为激发光源，构建了光纤型生化气体传感器(生物嗅探器)：在该传感器系统中，通过便携式光纤荧光系统检测乙醇蒸气中NADH作为乙醇脱氢酶(ADH)酶解产物的荧光变化，成功地测量了乙醇蒸气的浓度。该荧光嗅探系统可应用于其他气味化学物质(乙醛、甲醛、乳酸、丙酮酸、丁二酸等)的脱氢酶嗅探。更薄的…(B)三乙胺(TEA)是一种无色挥发性有机化合物，与气相中的氨一样，具有强烈的鱼腥味。利用含有黄素的单加氧酶3(FMO-3)研制了三乙胺(TEA)生物电子嗅探仪。茶叶生物滤器由Clark型溶氧电极和FMO-3固定化膜组成。为了放大生物传感器，采用了输出信号。(C)乙醛是一种特殊的恶臭物质，其最大允许浓度被ACGIH定义为25ppm。将碳电极和银/氯化银电极涂覆在滤纸上，将酶(ALDH)固定在碳电极上，构建了生物嗅探器。利用ALDH的底物专属性，在气相中使用ALDH嗅探器测定乙醛蒸气，校正范围为0.2~4.0ppm，具有良好的气体选择性。较少

A novel artificial arrayed nose system with drug metabolizing system was investigated with following steps,(a) optical fluorescent fiber with alcohol dehydrogenase for ethanol vapor,(b) biochemical sniffer with flavin containing monooxygenase as one of the drug metabolizing enzyme,(c) bio-sniffer chip with screen printed electrodes. The detailed results are described as follows.(a) The fiber optic type biochemical gas-sensor(bio-sniffer) was constructed by using NAD+ dependent dehydrogenase enzyme and exciting light source of UV-LED and portable spectrometer: In this sensor system, the concentration of ethanol vapor could be successfully measured by detecting the fluorescent change in NADH as ADH (alcohol dehydrogenase) enzymatic product by the portable fiber optic fluorometric system. The fluorescent sniffer system could be applied to other sniffers for other smell chemicals (acetaldehyde, formaldehyde, lactate, pyruvate, succinate, etc) using their dehydrogenase enzymes. The thinner … More fiber would be suitable for constructing the arrayed nose system for multi analyte in the smell.(b) Triethylamine (TEA) is a colorless volatile organic compounds with a strong fishy malodor, like ammonia, in the gas phase. A bioelectronic sniffer for triethylamine (TEA) was developed with a flavin-containing monooxygenase type 3 (FMO-3). The TEA biosniffer consisted of a Clarktype dissolved-oxygen electrode and an FMO-3 immobilized membrane. In order to amplify the biosensor output was applied. The FMO-3 biosniffer was used to measure TEA vapor.(c) acetaldehyde is one of the specific malodor substances, and its maximum permitted concentration is defined as 25 ppm by ACGIH. The bio-sniffer device was constructed by coating a carbon and Ag/AgCl electrodes on a filter paper, and immobilizing enzyme (ALDH) to the carbon electrode. The ALDH sniffer was used in the gas phase to measure acetaldehyde vapor with the calibration range from 0.2 to 4.0 ppm with good gas selectivity which was attributed to the substrate specificity of ALDH. Less