Toxicity test by use of 4-element hybrid strain derived from a Japanese wild rat NIG-III

近交系大鼠被广泛用于新开发药物的安全性试验，但近交系啮齿动物的遗传监测和遗传分析目前并不容易，因此，我们以日本野生大鼠nigi - iii为材料，开发了一种四元杂交毒理学工具。NIG-III菌株最初是在20世纪60年代在我们的设施中培养的。事实上，我们成功地在自交系DN、F344、nigi - iii和SHR中建立了大鼠多态微卫星标记。此外，为了验证4元杂交种遗传监测和遗传分析的准确性和有效性，我们从4个自交系DN、F344、nigi - iii和SHR中提取了一组杂交种，并测量了它们的出生体重。我们可以很容易地确定4元杂交种个体的基因型，但对它们的出生体重没有进行QTL分析。

Outbred rats are widely used in safety tests for newly developed drugs, however, genetic monitoring and genetic analysis in outbred rodents is not an easy task now Thus, as a tool for toxicity best, we developed a 4-element hybrid derived from a Japanese wild rat NIG-III. The strain NIG-III was originally raised in our facility in 1960s.Indeed, we successfully developed rat polymorphic microsatellite markers among inbred strains DN, F344, NIG-III and SHR. Furthermore, in order to demonstrate the accuracy and efficacy of genetic monitoring and genetic analysis in a 4-element hybrid strain, we generated a set of hybrid derived from 4 inbred strains DN, F344, NIG-III and SHR and measured their body weights at birth We could easily determined genotypes of the 4-element hybrid individuals, however, QTL analysis on their body weight at birth was unsuccessful.