Investigation of the Mechanism of the Production and the Evaluation as a Cell Culture Support of Polymer Porous Medium with Controlled 3-Dimensional Structure

本研究对超临界二氧化碳聚合法制备聚合物多孔介质进行了研究。1.聚合物多孔介质三维结构的控制研究在超临界二氧化碳中进行了苯乙烯和丙烯腈的交联聚合，并研究了聚合物多孔介质三维结构的控制方法。聚合反应在不同的压力、温度和浓度条件下进行。研究了聚合条件与多孔介质直径、一次粒子直径、比表面积和微孔直径分布的关系。2.聚合物多孔介质的生成机理推测聚合物在聚合场中的溶解度对聚合物多孔介质的生成机理有影响。基于聚合物从聚合溶液中沉淀出来的时间，聚合物的溶解度在…中被定量显示3.采用搅拌式超临界聚合反应器生产聚合物多孔介质。建立了生产苯乙烯多孔介质的工艺。考察了叶轮旋转次数对聚合反应速率和结构的影响。4.聚合物颗粒在多孔介质中的成型及表面处理、清洗，探讨了超临界二氧化碳处理聚合物颗粒在多孔介质上成型的方法。5.用聚合物多孔介质培养人前列腺癌细胞以聚苯乙烯和聚丙烯腈多孔介质作为培养载体进行人前列腺癌细胞培养。在所有的多孔介质中，细胞都在向多孔介质的内侧增加，证实了本研究中的聚合物多孔介质可以作为细胞的培养载体。较少

In this study, the production of the polymer porous medium by polymerization in supercritical carbon dioxide was investigated.1.Control of 3-dimensional structure of the polymer porous mediumThe cross linked polymerization of styrene and acrylics nitril was performed in supercritical carbon dioxide, and the control method of the 3-dimensional structure of the polymer porous medium was investigated. Polymerization was performed on various conditions of pressure, temperature and concentration. The relation between the polymerization conditions and the diameter of the porous medium, the diameter of a primary particle, specific surface area and the diameter distribution of fine pores was shown.2.Mechanism of the polymer porous medium productionIt is guessed that the solubility of the polymer in polymerizing field influences on the mechanism of polymer porous medium production. Based on time until polymer deposits out of polymerization solution, the solubility of polymer was shown quantitat … More ively, and the influence polymerization condition on structure was explained.3.Production of the polymer porous medium using stirred type supercritical polymerization reactorThe industrial production of the polymer porous medium using stirred type supercritical polymerization reactor was considered. The procedure which produces a styrene porous medium was established. The influence of the impeller rotations number on the rate of polymerization and structure was shown.4.Molding to polymer porous medium of the polymer particles and surface treatment, washing of the porous mediumThe method of molding polymer particles on the porous medium by treating with supercritical carbon dioxide was discussed. Furthermore, the surface treatment and washing of the polymer porous medium using supercritical carbon dioxide were studied.5.Cultivation of human prostatic cancer cell using polymer porous mediumThe human prostatic cancer cell culture using the polystyrene and the poly acrylics nitrile porous medium as a culture support was performed. In all porous mediums, the cell was increasing to the inside of the porous medium, and it was confirmed that the polymer porous medium in this study can use it as a cell a culture support. Less