The fumctional siRAs inducing flowering of chrysanthemum plants

菊花是一种必备的短日开花植物。因此，菊花的开花通常可以通过光周期来控制。然而，人们注意到，被CSVd感染的‘Piato’植物即使在长日照条件下也能自主开花。在这项研究中，我们阐明了CSVd感染与从不同大小的解剖茎顶端分生组织再生的‘Piato’植株自主开花的关系。在自然短日照条件下，含LPs的SAMS再生植株的开花时间比不含LP的SAMS再生植株早一个月。通过RT-PCR从这些早花植株中检测到CSVd。另一方面，在不含LP的SAMS再生植株中未检测到CSVd。在22：00-2：00的夜间光照模拟的长日照条件下，对感染CSVd和未感染CSVd的植株进行生长。所有感染CSVd的植物即使在长日照条件下也能自主开花；另一方面，未感染CSVd的菊花pl…更多的蚂蚁保持了营养生长。当无CSVd的植株通过嫁接到感染CSVd的根茎上接种CSVd时，即使在夜间照明下，它们也可以自主开花。在本研究中，结果表明CSVd可能控制菊花的质量发育过程，即开花，即它可以诱导菊花的自主开花。我们将siRNA表达载体导入‘I-69-8’植株，获得了几个转基因植株。载体1导入的1个品系和载体2导入的2个品系均表现出对CSVd的少量抗性。时程观察表明，在这些品系中，CSVd滴度在接种后升高，然后逐渐降低。载体1导入的一个品系在叶片中仅部分感染CSVd。因此，这些植物应该有一种siRNA，由受感染的CSVd分子和引入的基因之间的相互作用分解而产生。我必须检查植物中的siRNA产量和这些植物的开花时间。较少

Chrysanthemum is one of the obligative short-day flowering plants. Therefore, usually the flowering of chrysanthemum can be controlled by photoperiod. However, it was noted that 'Piato' plants infected by the CSVd flowered autonomously even under long-day conditions. In this study, we clarified the relationship between CSVd infection and the autonomous flowering of 'Piato' plants regenerated from different-sized dissected shoot apical meristems. Under natural short-day conditions, the flowering of plants regenerated from SAMs containing LPs was one month earlier than plants regenerated from LP-free SAMs. CSVd was detected from these early flowering plants by RT-PCR. On the other hand, CSVd was not detected in plants regenerated from LP-free SAMs. CSVd-infected and -free plants were grown under long-day conditions simulated by night-break lighting at 22: 00-2: 00. All CSVd-infected plants flowered autonomously even under long-day conditions; on the other hand, CSVd-free chrysanthemum pl … More ants maintained their vegetative growth. When the CSVd-free plants were inoculated with CSVd by grafting them to CSVd-infected root stocks, they flowered autonomously even under night-break lighting. In this study, results suggest that CSVd may control the qualitative development process, flowering; that is, it can induce the autonomous flowering of chrysanthemum.We introduced siRNA expressed vectors to 'I-69-8' plants and produced several transgenic plants. One line introduced by vector1 which has sense and antisence strand of 120bp CSVd sequences and two lines introduced by vector2 having 120bp CSVd sense sequence express a little bit of resistance to CSVd. And time course observation clarified that in these lines CSVd titers increase CSVd after inoculation and then decreases gradually. And one line introduced by vector1 was only partly infected by CSVd in a leaf. So these plants are supposed to have a siRNA produced by the break down by the interaction between infected CSVd molecules and introduced genes. I must check siRNA production in plants and the flowering time of these plants. Less