Obsevation of ordered biomolecules in solution by using CSI-MS

用冷喷雾电离(CSI)质谱仪分析了溶液中的各种生物分子，揭示了分子间的相互作用。例如，可以清楚地观察到蛋白质结合的水分子。该方法还证明了几个小分子与HAS之间存在较弱的分子间相互作用，建议采用高通量的质谱仪进行分析。结合DNA和RNA的绿茶儿茶素的CSI质谱分析有助于癌症预防的研究。另一方面，在分析双胍(BG)-苯甲酸(BA)相互作用的情况下，进行了关于溶液化学的相当基础的研究。用CSI方法在溶液和晶体中观察到这些分子间的相互作用形成基于氢键的聚集物种。在发展这种方法的过程中，我们发现了一种新的技术来解释反应中间体的动态溶液结构，这些中间体在反应容器中快速变化。我们将这种方法命名为RTS(反应跟踪系统)，并给出了几个分析结果。

Various biomolecules were analyzed in solution by using cold spray ionization (CSI) mass spectrometry to reveal the intermolecular interactions. Protein bound water molecules were clearly observed for example. This method also proved the weak intermolecular interaction between several small molecules and HAS, suggesting high throughput analysis using mass spectrometry. CSI mass spectrometric analysis for green tea catechins bound to DNA and RNA contributed to the study of cancer prevention. On the other hand, rather basic investigations with respect to solution chemistry was done in the case analyzing bisguanidine(BG)-benzoic acid (BA) interactions. These molecular interactions to form aggregated species based on hydrogen bonding were observed using CSI method in solution as well as in the crystal. In developing this method, we found a new technique to elucidate dynamic solution structure of reaction intermediate, which are rapidly transforming in the reaction vessel. We named this method RTS (reaction tracking system) and several analytical results were shown.