Anti-oxidative Stress Function and Utilization of Germed Sesame Seed

芝麻木脂素的健康促进作用已被阐明，最近对木脂素的关注正在增加。本文对食品中木脂素类化合物的高效液相色谱-紫外分光光度法和荧光分光光度法进行了研究。与固定正相（硅胶）系统相比，反相负相（ODS）系统的分离效果良好。用荧光检测器检测到芝麻素和芝麻林素的含量是紫外检测器的千倍以上，当白色芝麻种子发芽后，检测到芝麻素和芝麻林素的含量是紫外检测器的千倍以上，当芝麻种子发芽后，检测到芝麻素和芝麻林素的含量是紫外检测器的千倍以上。（关东1号）种子萌发后，芝麻素和芝麻林素含量降低，极性化合物含量增加，甲醇提取物清除DPPH自由基能力和SOD活性增强。在光谱学证据的基础上，在萌发过程中含量增加的六种主要化合物中，一种被鉴定为咖啡酸糖苷酯（毛蕊花糖苷），其他被鉴定为已知的木脂素二-和三-葡萄糖苷。毛蕊花糖苷是已知的糖苷，但首次从发芽芝麻中分离得到。采用比色法测定其清除DPPH自由基的活性，发现只有类叶升麻苷具有较强的清除DPPH自由基的活性。在SOD活性方面，毛蕊花糖苷及其苷元（咖啡酸）在等摩尔浓度下表现出比曲洛昔更强的活性。

The health-promoting effect of sesame lignans has been clarified, and the concern about lignans is recently increasing. Herein the method of quantification of lignans in food using HPLC withUV spectrometer or fluorometer was examined. The separation was good with a reverse negative-phase (ODS) system in comparison with a fixed positive-phase (silica) system. The possibility of measuring lignans in the serum of the rat orally administered germinated sesame seed was examinedSesamin and sesamolin were detected thousand times higher level than UV detector by fluorescence detector.When white sesame seed (Kanto No.1) germinated, contents of sesamin and sesamolin were decreased and the contents of polar compounds were increased and DPPH radical scavenging activity and SOD activity in methanol extract were enhanced. On the basis of spectroscopic evidence, among the six main compounds whose contents increased during germination, one was identified as caffeic acid glycoside ester (acteoside) , and the others were identified as known lignan di- and tri- glucosides. Acteoside are already known glycoside but isolated from germinated sesame seed for the first time. On the DPPH radical scavenging activity by colorimetric method, only acteoside showed strong activity. On the SOD activity, acteoside and its aglycon (caffeic acid) showed stronger activity than trolox at the equal molar concentration.