Development of Hg(II)-trapping technology based on the physicochemical properties of the T-Hg(II)-T base-pair

最近，粮农组织和世卫组织警告说，大型鱼类（特别是鱼类）体内积累的汞含量已达到对胚胎有害的水平。为了减少鱼类体内的汞积累，降低环境汞的浓度（总量）是很重要的。为此，已经提出了大量从环境中捕获汞（II）的技术，例如用碘化物沉淀(r)，用edta相关化合物或半胱氨酸修饰的树脂螯合汞（II），以及细菌捕获汞（生物修复）。然而，这些技术并不是针对Hg（II）离子的。在本项目中，我们提出了一种新的汞（II）捕获技术，该技术对汞（II）具有高度特异性。该技术依赖于我们小组的知识，即汞（II）被DNA双链中T -T错配的胸腺嘧啶(T)残基捕获（J. Am Chem Soc）。浙江农业学报，2006,28(2):2172-2173。因此，该基金的获得者（Tanaka）假设，通过使用经oligo-T修饰的树脂，将会…更多的工作作为汞（II）捕获装置。然后，他合成了低聚t修饰的聚苯乙烯树脂，并用ICP光谱检测了其汞（II）捕获效率。结果表明，该低聚t改性聚苯乙烯树脂（Hg（II）捕集装置）在溶液中存在或不存在其他金属组分时均能有效地捕集Hg（II）。更有趣的是，这种汞（II）捕获装置专门捕获了汞（II），而没有捕获任何其他金属阳离子。此外，获奖者还进行了T-Hg(II) T碱基对的结构测定和物理化学表征，以促进更有效的Hg（II）捕获装置的开发，并为它们阐明合适的序列。特别是用^15N核磁共振法测定了T- hg (II) T碱基对的化学结构。， 2007, 129, 244-245.)在全球范围内对核酸-金属相互作用领域产生了影响，并被邀请作为。一本书章节的作者（N. Hadjiliadis和E. Sletten），“金属配合物- DNA相互作用”，Blackwell出版，牛津，英国)和个人帐户（道尔顿交易）。综上所述，获奖者揭示了T-Hg(II) T碱基对的基本性质，并基于T-Hg(II) T碱基对的基础科学发明了一种Hg（II）捕集装置。少

Recently, FAO and WHO warned that the amount of mercury accumulated in large fishes (especially in tune) became a toxic level against embryos. In order to reduce mercury accumulated in fishes, it is important to reduce the concentration (total amount) of environmental mercury. For this purpose, considerable numbers of techniques to capture Hg(II) from environment have been proposed, such as precipitation with iodide (r), chelation of Hg(II) by resins modified with EDTA-related compounds or cysteine, and bacterial trapping of mercury (bio-remediation). However, these techniques are not specific for Hg(II) ions. In this project, we propose a new technology for a Hg(II)trapping which is highly specific for Hg(II). This technique rely on a knowledge in our group that Hg(II) is captured by thymine (T) residues in T -T mismatches in DNA duplexes (J. Am Chem Soc., 2006, 128, 2172-2173.). Therefore, the awardee of this grant (Tanaka) postulated that by using resins modified with oligo-T would … More work as a Hg(II)trapping device. He then synthesized the oligo-T-modified poly-stylene resin, and examine its Hg(II)-trapping efficiency with ICP spectroscopy. It was found that this oligo-T modified poly-stylene resin (Hg(II) trapping device) efficiently trapped Hg(II) from solution in the presence or absence of other metal rations. More interestingly, this Hg(II)trapping device specifically captured Hg(II) without trapping any other metal cations significantly. In addition, the awardee performed structure determination and physicochemical characterizations of T-Hg(II) T base-pairs, in outer to facilitate developments of more efficient Hg(II) trapping devices and to clarify appropriate sequences for them. Especially, the determination of the chemical structure of T-Hg(II) T base-pairs with ^15N NMR (J. Am Chem Soc., 2007, 129, 244-245.) gave impact to the field of nucleic acids-metal interactions in world-wide manner, and the awardee was invited as. A writer for a book chapter (N. Hadjiliadis and E. Sletten Eds., "Metal Complexes - DNA Interactions", Blackwell Publishing, Oxford, UK.) and a personal account (Dalton Transactions). In conclusion, the awardee revealed the fundamental properties of the T-Hg(II) T base-pan and created a Hg(II)-trapping device based on basic sciences of the T-Hg(II) T base-pairs. Less