Construction of the glycerol dehydratase (reuterin-producing) gene knockout mutant Lactobacillus reuteri and probiotics evaluation

鲁氏乳杆菌是一种异源发酵乳酸菌，自然存在于人类和其他动物的肠道中。鲁氏乳杆菌的益生菌作用被认为在很大程度上与甘油厌氧代谢过程中产生广谱抗菌化合物鲁菌素有关。我们测定了产reurin的L.reuri JCM 1112^T的全基因组序列。reuriJCM 1112^T有一个独特的58个与reurin和钴胺(维生素B)生物合成相关的基因簇。~-lt；13&gt；G_3-甘油的二维核磁共振(2D-核磁共振)结果表明，罗氏乳杆菌JCM 1112^T在体内能够将甘油转化为尿嘧啶，证实了罗氏乳杆菌JCM 1112^T在肠道中产生尿嘧啶的能力。鉴于甘油被证明天然存在于粪便中，获得的产生reurin和钴胺的能力是一种适应性进化反应，可能有助于L.reuri的益生菌特性。

Lactobacillus reuteri is a heterofermentative lactic acid bacterium that naturally inhabits the gut of humans and other animals. The probiotic effects of L. reuterihave been proposed to be largely associated with the production of the broad-spectrum antimicrobial compound reuterin during anaerobic metabolism of glycerol. We determined the complete genome sequences of the reuterin-producing L. reuteri JCM 1112^T. L. reuteriJCM 1112^T has a unique cluster of 58 genes for the biosynthesis of reuterin and cobalamin (vitamin B_<12>). Two-dimensional nuclear magnetic resonance (2D-NMR) with ^<13>G_3-glycerol demonstrated that L. reuteri JCM 1112^T could convert glycerol to reuterin in vivo, substantiating the potential of L. reuteri JCM 1112^T to produce reuterin in the intestine. Given that glycerol is shown to be naturally present in feces, the acquired ability to produce reuterin and cobalamin is an adaptive evolutionary response that likely contributes to the probiotic properties of L. reuteri.