In vivo analysis of the gene expression of L.reuteri under the presence of glycerol

罗伊氏杆菌以甘油为底物生成3-羟基丙醛（3-HPA）。脱水型3-HPA从细胞中释放出来，水合型3-HPA二聚体在肉汤中具有可逆性（HPA体系）。所有类型的3-HPA通常以reuterin命名。没有罗伊氏杆菌在动物胃肠道中产生罗伊氏素的报道。因此，采用罗伊氏Lb.reuteri JCM 1112联合无菌小鼠，采用^<13> c_3 -甘油二维核磁共振（NMR）在体内检测小鼠盲肠内容物中的3-HPA。与未摄入甘油组相比，与^<13> c_3 -甘油产生3-HPA相关的基因有意向性表达。特别是甘油转运基因pduF的表达与不摄入甘油组有显著差异。目前还没有从益生菌中检测到这种体内抗菌作用的报道。我们首次报道了罗伊氏杆菌产生的3-HPA的体内检测，证明了益生菌在哺乳动物胃肠道中具有抗菌作用。

Lb.reuteri generates 3-hydroxy propionaldehyde (3-HPA) as a substrate with glycerol. The dehydration type of 3-HPA is released from the cells and the hydration type or the dimer of 3-HPA with the reversibility (HPA system) in broth. All the types of 3-HPA are named generally with reuterin. There are no report that Lb. reuteri produced reuterin in animal gastrointestinal tract. Therefore, Lb.reuteri JCM 1112 associated gnotobiotic mouse was used, and 3-HPA in the mouse cecal contents was detected in vivo by the two-dimensional nuclear magnetic resonance (NMR) using ^<13>C_3-glycerol. The genes which associated with 3-HPA production from ^<13>C_3-glycerol was expressed in intentionality comparison with no glycerol intake group. Particularly, the expression of pduF which was a glycerol transporter gene recognized the significant difference with the group which take in no glycerol. here was no report that detected from probiotics such an antibacterial in vivo. It was proved that probiotics produced antibacterial in the gastrointestinal tract of the mammal according to our first report that in vivo detection of 3-HPA which was produced by Lb. reuteri.