In vivo analysis of the gene expression of L.reuteri under the presence of glycerol

罗伊氏乳杆菌与甘油生成3-羟基丙醛（3-HPA）作为底物。在发酵液中，3-HPA的脱水型和水合型或二聚体具有可逆性（HPA系统）。所有类型的3-HPA都以reuterin命名。没有报道说LB。reuteri在动物胃肠道产生reuterin。因此，本研究采用罗伊氏乳杆菌JCM 1112共生菌系小鼠，用~ 13C_3-甘油二维核磁共振（NMR）技术，在体内检测了小鼠盲肠内容物中的3-HPA<13>。与<13>不摄入甘油组相比，与^ C_3-甘油产生3-HPA相关的基因在意向性表达。特别是甘油转运蛋白pduF的表达与不摄入甘油的组有显著差异。目前还没有从益生菌中检测到这样的体内抗菌作用的报道。本课题组首次报道了乳酸杆菌产生的3-HPA在哺乳动物胃肠道中的体内检测结果，证明了益生菌在哺乳动物胃肠道中具有抗菌作用。reuteri。

Lb.reuteri generates 3-hydroxy propionaldehyde (3-HPA) as a substrate with glycerol. The dehydration type of 3-HPA is released from the cells and the hydration type or the dimer of 3-HPA with the reversibility (HPA system) in broth. All the types of 3-HPA are named generally with reuterin. There are no report that Lb. reuteri produced reuterin in animal gastrointestinal tract. Therefore, Lb.reuteri JCM 1112 associated gnotobiotic mouse was used, and 3-HPA in the mouse cecal contents was detected in vivo by the two-dimensional nuclear magnetic resonance (NMR) using ^<13>C_3-glycerol. The genes which associated with 3-HPA production from ^<13>C_3-glycerol was expressed in intentionality comparison with no glycerol intake group. Particularly, the expression of pduF which was a glycerol transporter gene recognized the significant difference with the group which take in no glycerol. here was no report that detected from probiotics such an antibacterial in vivo. It was proved that probiotics produced antibacterial in the gastrointestinal tract of the mammal according to our first report that in vivo detection of 3-HPA which was produced by Lb. reuteri.