In vivo analysis of the gene expression of L.reuteri under the presence of glycerol

Lb.reuri与甘油一起产生3-羟基丙醛(3-HPA)作为底物。3-HPA的脱水类型是从细胞中释放出来的，而3-HPA的水合类型或二聚体是在发酵液中具有可逆性的(HPA系统)。所有类型的3-HPA都一般用reurin命名。没有关于lb的报道。Reuri在动物胃肠道中产生reurin。因此，以Lb.reuri JCM 1112联合灵芝属小鼠为研究对象，利用~(13)&gt；C_3-甘油的二维核磁共振技术对小鼠盲肠内容物中的3-HPA进行了体内检测。与不摄入甘油组相比，与~(13)&GT；C_3-甘油产生3-HPA相关基因有有意表达。特别是，甘油转运蛋白基因pduF的表达证实了与不摄入甘油组的显著差异。在体内还没有从益生菌中检测到这种抗菌剂的报道。根据我们的第一个报告，体内检测到由Lb产生的3-HPA，证明了益生菌在哺乳动物的胃肠道中产生了抗菌作用。路易。

Lb.reuteri generates 3-hydroxy propionaldehyde (3-HPA) as a substrate with glycerol. The dehydration type of 3-HPA is released from the cells and the hydration type or the dimer of 3-HPA with the reversibility (HPA system) in broth. All the types of 3-HPA are named generally with reuterin. There are no report that Lb. reuteri produced reuterin in animal gastrointestinal tract. Therefore, Lb.reuteri JCM 1112 associated gnotobiotic mouse was used, and 3-HPA in the mouse cecal contents was detected in vivo by the two-dimensional nuclear magnetic resonance (NMR) using ^<13>C_3-glycerol. The genes which associated with 3-HPA production from ^<13>C_3-glycerol was expressed in intentionality comparison with no glycerol intake group. Particularly, the expression of pduF which was a glycerol transporter gene recognized the significant difference with the group which take in no glycerol. here was no report that detected from probiotics such an antibacterial in vivo. It was proved that probiotics produced antibacterial in the gastrointestinal tract of the mammal according to our first report that in vivo detection of 3-HPA which was produced by Lb. reuteri.