New Type of Polyphenolic Compounds Targeting for Healthy Food-stuff

以往对海鞘地上部分和地下部分的研究已分离出5种木脂素、10种酯化糖苷以及一种著名的化合物咖啡酸。在本研究中，我们使用脂氧合酶和透明质酸酶两种体外检测系统检测了这16个化合物的酶抑制活性，认为酚性官能团的生物活性归因于它们的酚性官能团。该功能对人类健康最重要和最广为人知的作用可能是抗氧化活性。像酚这样的抗氧化剂可以清除人体内的自由基中间体，并可以通过自身氧化来阻止其他氧化反应。苯酚化合物对人类健康的其他重要作用被认为是一些酶抑制活性。为了验证这一预期的活性，我们分别检测了从大白菜中分离得到的化合物对脂氧合酶和透明质酸酶活性的抑制作用。我们得出结论：就透明质酸酶的抑制活性而言，1，2-二-O-咖啡酰基葡萄糖的活性最强。另一方面，在脂肪氧合酶抑制活性方面，1-O-咖啡酰基-6-O-没食子酸葡萄糖的活性最强，因此，尽管有必要验证这种植物的毒性，但毫无疑问，这种寄生植物有可能成为一种健康的食品。

Previous studies on the aerial part and under-ground part of Balanophora japonica have resulted in the isolation of five lignans, ten kinds of esterified glucoside together with a well-known compound, caffeic acid. In this study, we examined the enzyme inhibitory activities of these 16 compounds using two in vitro assay systems with lipoxygenase and hyaluronidase.It is believed that the biological activities of phenolic functional groups are attributable to their phenolic functional groups. The most important and well-known role of the function for human health is probably the antioxidant activity. Antioxidants like phenols can remove radical intermediate in human body and can prevent other oxidation reactions by oxidizing themselves. Other important role of phenol compound for human health is thought to be some enzyme inhibitory activities. For the purpose of verification of this expected activities, we examined the inhibitory effect of some isolated compounds from B. japonica on lipoxygenase and hyaluronidase activities, respectively.We conclude that with respect to the inhibition activity of hyaluronidase, 1,2-di-O-caffeoyl glucose had the most potent activity. On the other hand, in the case of lipoxygenase inhibitory activity, 1-O-caffeoyl 6-O-galloyl glucose had the most potent activity.Thus, although it is necessary to verify any kind of toxicity for this plant, there is no doubt that this parasitic plant, Balanophora japonica, would have the possibility of being a healthy food-stuff.