Elucidation of molecular structure of lipid peroxides as oxidative suns markers and and application of those to legal medicine

1. 采用LC/ESI-MS和LC/ESI-MS对人血浆中羟基磷脂酰胆碱（PC 16:0 / 18:2-OOH）、羟基磷脂酰胆碱（PC 16:0 / 18:2-OH, PC- oh）、羟基磷脂酰胆碱（PC 16:0 / 18:2-OH, PC- oh）、环氧羟基-PC、氧基-PC、三羟基-PC的分子结构进行了鉴定。还鉴定出氢过氧溶磷脂酰胆碱（18:2-OOH）和氧化溶磷脂酰胆碱（oxo-18:2）。18:2、20:4、16:0、18:1和18:0被鉴定为溶磷脂酰胆碱的分子种。(1)大鼠小肠酒精障碍及大豆苷的抗氧化作用急性乙醇喂养诱导大鼠小肠胆固醇过氧化氢和氧化甾醇积累，提示乙醇负荷增加了氧化应激。事先装上大原就取消了这种效果。(2)大鼠禁食应激大鼠禁食后第1天的氧化应激强于第2天。(3)放骨针缺血-再灌注引起的氧化应激：放骨针缺血-再灌注引起的氧化应激不仅在腓肠肌中增强，而且在肾脏和肝脏中也有增强。(4) d -半乳糖胺对肝脏的损害，以及抗氧化负载d -半乳糖胺诱导过氧化氢胆固醇和氧化甾醇在大鼠肝脏、脑和腓肠肌的积累，提示d -半乳糖胺的氧化应激依赖于器官或组织。

1. Identification of molecular structure of hydroperoxy phosphatidylcholine (PC 16:0 / 18:2-OOH), phosphatidylcholine hydroxide (PC 16:0 / 18:2-OH, PC-OH)Hydroperoxy phosphatidylcholine (PC 16:0 / 18:2-OOH), phosphatidylcholine hydroxide (PC 16:0 / 18:2-OH, PC-OH), epoxyhydroxy-PC, oxo-PC, trihydroxy-PC were identified in human plasma by LC/ESI-MS and LC/ESI-MS-MS. Hydroperoxy lyso-phosphatidylcholine (18:2-OOH) and lyso- phosphatidylcholine oxide (oxo-18:2) were also identified. 18:2, 20:4, 16:0, 18:1, and 18:0 were identified as molecular species of lyso-phosphatidylcholine.2. Oxidative stress identified by oxisterols as a marker(1) Alcoholic disorder in rat small intestine and anti-oxidative effect of daizeinAcute ethanol feeding induced accumulation of cholesterol hydroper-oxide and oxysterols in rat small intestine, suggesting that ethanol loading increased oxidative stress. Loading of Daisein in advance canceled such effects.(2) Fasting stress in ratOxidative stress due to fasting was stronger in the first day after fasting than in the second day in rat.(3) Oxidative stresss due to ischemic-reperfusion in turniquet-releaseThe enhanced oxidative stresss due to ischemic-reperfusion in turniquet-release mice were observed not only in the gastrocnemius muscles but also in the kidneys and liver.(4) Liver disorder by D-galactosamine and the effect of anti-oxidantD-galactosamine loading induced accumulation of cholesterol hydroperoxide and oxysterols in rat liver, brain and gastrocnemius muscles, suggesting that oxidative stress by D-galactosamine depend on organs or tissues.