Studies on vertebrate development by the transposon-mediated Gal4 enhancer trap method

我们成功地开发了Gal 4增强子陷阱和基因陷阱方法与Tol 2转座因子，并产生了1,000多个转基因鱼行，表达Gal 4在空间和时间上的限制性方式在胚胎发育。利用这些转基因鱼，我们揭示了器官发生的机制，包括侧线，淋巴系统，透镜，视网膜和脊索。此外，我们分析了脊髓感觉和中间神经元和嗅觉感觉神经元的特定子集的抑制引起的行为异常。此外，我们通过钙成像可视化了运动回路的放电。因此，我们为脊椎动物的发育生物学和神经科学研究奠定了基础。

We successfully developed the Gal4 enhancer trap and gene trap methods with the Tol2 transposable element, and generated more than 1,000 transgenic fish lines that express Gal4 in spatially and temporally restricted fashions during embryonic development. By using these transgenic fish, we disclosed mechanisms of organogenesis including the lateral line, lymphatic system, lens, retina and notochord. Further, we analyzed behavioral abnormalities caused by inhibition of spinal sensory- and inter-neurons and a specific subset of the olfactory sensory neurons. Furthermore, we visualized firing of motor circuits by calcium imaging. Thus, we established the basis for studies of developmental biology and neuroscience in a vertebrate.