Regulation with secretory signals and neurotrophic factors of salivary secretion-related protein expression

唾液腺分泌蛋白含量受多种因素调控。我们研究了分泌蛋白在分泌刺激和自主神经系统下的表达机制，得到了以下结果异丙肾上腺素（ISP）对唾液腺蛋白有调节作用，而氨基酚（CCh）和地塞米松对唾液腺蛋白无明显调节作用用ISP处理离体唾液组织可诱导c/ ebps的表达唾液腺泡细胞的培养诱导了退行性改变，我们不能确定细胞通讯对培养细胞蛋白表达控制的贡献去神经支配未引起唾液腺重量和淀粉酶含量的显著变化，但进一步用ISP处理可引起组织重量和新蛋白的表达。ISP降低了淀粉酶含量，提高了mRNA水平。ISP降低了Akt、mTOR、S6k和p27，但增加了PCNA。去神经支配倾向于降低S6k，但其他蛋白没有任何变化。ISP和去神经支配降低了S6k和p27，但增加了pcna。mTOR阻滞剂雷帕霉素（Rapamycin, Rap）增强了腺体重量的降低。Rap降低了过氧化物酶含量，但没有降低淀粉酶含量。唾液蛋白表达可能通过Akt-mTOR-S6k信号系统调节，并伴有分泌和自主神经刺激。

Secretory protein contents in the salivary glands are regulated with various factors. We investigated the expression mechanism of secretory proteins with secretory stimulation and autonervous system, and obtained the following results.1 Proteins in the salivary glands were modulated with isoproterenol (ISP) application, but not markedly with carbachol (CCh) and dexamethasone.2 Treatment of isolated salivary tissues with ISP induced the expression of c/EBPs.3 Culture of salivary acinar cells induced the degenerative changes, and we could not identified the contribution of cell communication to the protein expression control in cultured cells.4 Denervation did not induce marked changes in salivary gland weight and amylase content, but further treatment with ISP induced the tissue weight and new protein expression. ISP decreased amylase content but increased their mRNA levels. ISP increased peroxidase content, and denervation decreased it5 ISP decreased Akt, mTOR, S6k, and p27, but increased PCNA. Denervation tended to decrease S6k, but did not any changes in other proteins. ISP and denervation decreased S6k and p27, but increased PCNA.6 Rapamycin (Rap), blocker of mTOR, potentiated the decrease in the gland weight. Rap decreased peroxidase content, but not amylase content. Salivary protein expression might be regulated through Akt-mTOR-S6k signaling system with secretory and autonomic nervous stimulation.