Studies on iterative type I polyketide synthases

聚酮酶是参与构建聚酮化合物基本碳骨架的重要酶。在这项研究中，结构和反应控制机制的迭代I型PKSs进行了研究。在6-甲基水杨酸合成酶上，发现了一个新的催化结构域，命名为“硫酯水解酶”结构域，它参与产物的释放。此外，新的方面的反应控制机制的非还原型I型PKSs，还原型I型PKSs和PKS-NRPS（非核糖体肽合酶）被确定。

Polyketide synthases (PKSs) are important enzymes involved in the construction of basic carbon skeletons of polyketides. In this study, structure and reaction control mechanisms by iterative type I PKSs were investigated. On the 6-methylsalicylic acid synthase, a new catalytic domain named "Thioester Hydrolase" domain was identified which is involved in the product release. In addition, new aspects on the reaction control mechanisms of non-reducing type I PKSs, reducing type I PKSs, and PKS-NRPS (non-ribosomal peptide synthase) were identified.