Subcellular co-localisation of interacting proteins that control maturation in mammalian eggs

哺乳动物卵子（卵母细胞）在受精前的最终成熟涉及蛋白激酶和磷酸酶之间的级联相互作用，这些蛋白质在细胞质和细胞核之间穿梭，以及微管的组装和拆卸。在这个项目中，我们提出了参与卵母细胞成熟的相互作用蛋白在卵母细胞的亚细胞位点共定位，严格的时间和空间方式。卵母细胞中蛋白质的共定位被认为是确保有时间限制的细胞事件协调和卵母细胞受精和早期胚胎发育的适当准备的基本机制。这对体外受精和核移植克隆具有重要意义。

The final maturation of mammalian eggs (oocytes) before fertilisation involves a cascade of interactions between protein kinases and phosphatases, the shuttling of these proteins between cytoplasm and nucleus, and microtubule assembly and disassembly. In this project we have proposed that interacting proteins involved in oocyte maturation are co-localised at subcellular sites in oocytes, in a strict temporal and spatial manner. The co-localisation of proteins in oocytes is considered a fundamental mechanism that ensures coordination of time-bound cellular events and proper preparation of oocytes for fertilisation and early embryo development. There are important implications for IVF and cloning by nuclear transfer.