Fabrication of carbonate apatite bone substitute which can be created as an arbitrary shape

本研究的目的是制备低结晶度的碳酸磷灰石块作为骨替代材料。首先，将凝固石膏浸泡在几种磷酸盐水溶液中，水热处理后，凝固石膏转化为低结晶度的碳酸盐磷灰石，并保持其原始形状。对制备的材料进行了体外生物相容性研究。结果表明，该样品对成骨样细胞和破骨细胞均无不良影响。结果，检测到骨直接沉积到样本上。多核细胞形成的Howship's陷窝假设在标本周围有一些与成骨细胞形态相似的细胞，我们认为这是一种有用的骨填充材料制备方法，因为它可以制备出与骨缺损形状相同的低结晶碳酸磷灰石。

The aim of this study was to fabricate the low-crystalline, carbonate apatite block as a bone substitute material. First of all, set gypsum was immersed in several kinds of phosphate aqueous solution.After hydrothermal treatment, set gypsum converted to low-crystalline carbonate apatite retaining its original shape. Analysis of fabricated specimen showed that component is similar to that of natural bone except strength.Investigation of the biocompatibility of the fabricated specimen in vitro was performed. As a result, it was demonstrated that specimen showed no adverse effect on either osteoblast-like cells or osteoclasts.Following the in vitro experiment, animal experiment was performed. As a result, direct bone deposition to the specimen is detected. Howship's lacuna formed by multinucleated cells suppose There are some cells which have a similar morphology of osteoblast around the specimen.We concluded that this is a useful bone filler fabrication method since low-crystalline carbonate apatite same as the shape of bone defect could be fabricated.d to be osteoclasts, are observed around the specimen.