Examination and pursuit of autologous nerve transplantation using dental pulp

我们研究了在异种移植模型中牙髓是否可以作为移植物的候选者。从正畸治疗中拔除的活牙中获得，然后用冷冻和解冻方法处理以杀死活细胞和降低抗原性。将牙髓放入壳聚糖管中，移植到切断的坐骨神经中。移植后8周，显微镜下可见明显的再生神经，但小神经束局限性较强，数量较少。抗神经丝和抗S-100抗体也表明再生，表明牙髓中剩余的雪旺细胞基底层充当支架。两者合计，牙髓神经可以是一个很好的候选人，神经移植在异种移植模型。

We investigated whether dental pulp could be a candidate for graft in a xeno-graft model. It was obtained from the vital extracted tooth for orthodontic treatment, and then treated with freezing and thawing method for killing viable cells and for reducing antigenicity. After putting the dental pulp into chitosan tube, it was transplanted into transected sciatic nerve. Eight weeks after transplantation, regenerated nerve was obviously observed on microscopic investigation, although the mini fascicles were localized and the number of them was small. Anti-neurofilament and Anti-S-100 antibody indicated regeneration as well, suggesting that remaining Schwann cell basal laminae in the pulp acted as a scaffold. Taken together, dental pulp nerve could be a good candidate for nerve grafting in a xeno-graft model.