Establishment of a method inducing embryonic stem cells to differentiate to hematopoietic stem cells (HSC) for therapeutic HSC transplantation

本研究旨在建立一种不需要动物细胞和血清的诱导人胚胎干细胞向造血干细胞分化的方法，为临床应用奠定基础。简而言之，我们首先将人ES细胞转移到明胶包被的培养皿上，并用5%人血小板裂解液或含血清的培养基培养，以诱导其分化为基质细胞。在无血清条件下，将人ES细胞与基质细胞共培养2周，获得多能造血细胞，从而成功地建立了一种无血清、无动物细胞诱导人ES细胞向多能造血细胞分化的新方法。

We challenged to establish a method inducing human embryonic stem (ES) cells to differentiate into hematopoietic stem cells without animal cells and serum for their clinical application. In brief, we first transferred human ES cells onto gelatin-coated culture dishes and cultured with 5% human platelet lysate or serum-containing media to induce their differentiation into stromal cells. Then, human ES cells were cocultured with the estabshished stromal cells under animal serum-free condition for 2 weeks to generate multipotent hematopoietic cells.Thus, we succeeded the establishment of a novel method inducing human ES cells to differentiate into multipotent hematopoietic cells without anima cells and seru,.