Disc repair with transplantation of activated nucleus pulposus cells-how to preserve the cells and its safety-

本研究的目的是评价活化髓核细胞冻存后的安全性和生物学特性。以动物模型和人体材料为材料，比较了正常激活的髓核细胞与冻存2周后激活的髓核细胞和冻存2周后髓核组织本身两种冻存方法的安全性和性质。在动物和人体材料中，这三个亚组之间的安全性、髓核细胞增殖或细胞基质潜力无显著差异。我们证实了深低温保存髓核对修复各种椎间盘病变是有用的。

The purpose of this study is to evaluate the safety and property of the activated nucleus pulposus cells after the cryopreservation. With the animal model and human material, we compared safety and property of the nucleus pulposus cells, ordinarily activated, to those in two subgroups on cryopreservation method ; 1) activated nucleus pulposus cells after cryopreservation for two weeks and 2) nucleus pulposus tissue itself after cryopreservation for two weeks. There was no significant difference amongst those three subgroups on the safety, the proliferation of the nucleus pulposus cells or the potentiality for the cell matrix in both animal and human materials. We confirmed cryopreservation of the nucleus pulposus is useful to repair various disc lesions.