Specific degradation of peptides with hydrogen radical and its application to protein chemistry

以添加的苯硫醇为释氢剂，在水溶液中进行了多肽的超声水解性研究。超声水解肽的特点不同于传统的酸水解，即在脯氨酸残基上发生裂解，并作为副反应脱水。超声裂解结合硫醇加成使测定多肽的氨基酸序列成为可能。在真空条件下的紫外激光光子辐照实验中，发现了新的氢原子释放剂。

The ultrasound sonolytic hydrolysis of peptides was performed with added benzenethiol as a hydrogen-releasing reagent in aqueous solution. The ultrasound hydrolysis of peptides differed from that due to conventional acid hydrolysis in characteristics, i.e., cleavage at proline residue and dehydration as a side reaction. The sonolysis combined with the thiol addition made it possible to determine the amino-acid sequence of peptides. Furthermore, new reagent for releasing hydrogen atoms was found out in the experiment of the irradiation of ultraviolet laser photon in vacuum condition.