Studies on the physiological functions of phospholipases A2

在PC12细胞中，神经生长因子(NGF)激活MAPK的磷酸化并诱导神经元分化，溶血磷脂酰胆碱(LPC)能有效地促进这一过程。没有其他被测试的溶血磷脂显示出类似的活性。发生在其他生长因子(如表皮和成纤维细胞生长因子)下游的MAPK激活/磷酸化不受LPC的影响。LPC能显著增强NGF诱导的TrkA自身磷酸化，提示LPC的作用是NGF-TrkA信号通路的特异性作用。在丝状真菌米曲霉中，AoPlaA定位于线粒体。亚细胞分裂实验表明AoPlaA定位于线粒体膜内间隙。磷脂分析表明，在AopaA高表达和缺失菌株中，磷脂酰乙醇胺和心磷脂分别减少和增加。

In PC12 cells, nerve growth factor(NGF) activates MAPK phosphorylation and induces neuronal differentiation, which is potently enhanced by lysophosphatidylcholine(LPC). No other lysophospholipids tested displayed similar activity. MAPK activation/phosphorylation that occurs downstream of other growth factors, such as epidermal and fibroblast growth factors, was not affected by LPC. TrkA autophosphorylation elicited by NGF was markedly enhanced by LPC, suggesting that the effect of LPC was specific to NGF-TrkA signaling pathway. AoPlaA in the filamentous fungus Aspergillus oryzae localizes to mitochondria. Subcellular fractionation experiment showed that AoPlaA localized to the intramembrane space of mitochondria. Phospholipid analysis demonstrated that phosphatidylethanolamine and cardiolipin was decreased and increased in AoplaA-overexpressing and-deleted strains, respectively.