Identification of novel essential factors responsible for class switch recombination of immunoglobulin genes.

激活诱导胞苷脱氨酶（AID）是免疫球蛋白基因类开关重组的关键。将DNA损伤引入免疫球蛋白基因的开关区需要AID。然而，一个重要的问题仍然是如何导致双链DNA在开关区切割。基于AID编辑mRNA编码的蛋白质产生DNA切割的假设，我利用逆转录病毒cDNA文库在aids缺陷B细胞中进行了类开关重组的救援实验。现在对几个候选基因进行了分析，以确认其在类开关重组中的作用。

Activation-induced cytidine deaminase (AID) is essential for class switch recombination of immunoglobulin genes. AID is required to introduce DNA lesions into switch region of immunoglobulin genes. However, an important question remains concerning how AID leads to double-strand DNA cleavage in switch region. Based on the hypothesis that a protein coded by the mRNA edited by AID generates DNA cleavage, I performed a rescue experiment for class switch recombination in AID-deficient B cells using retroviral cDNA library. Several candidate genes are now analyzed to confirm its role in class switch recombination.