The role of micro RNA and methylated gene in multistep genesis and prognosis of multiple myeloma

我们在骨髓瘤细胞系和骨髓瘤患者中检测到甲基化的WWOX启动子，但在MGUS患者中未检测到甲基化。在骨髓瘤患者、MGUS和细胞系中均检测到WWOX mRNA的异常转录。部分患者纯化浆细胞中微rna miR15a、15b、16表达水平极低或缺失。MiR 15a、15b、16在MM浆细胞中的表达水平低于MGUS。相反，MM的浆细胞中Dnmts和Mcl-1水平高于MGUS。

We detected methylated WWOX promoter in myeloma cell lines, myeloma patients but not in MGUS patients. The aberrant transcript of WWOX mRNA was detected in myeloma patients, MGUS and cell lines. The expression level of micro-RNA miR15a 15b, 16 were very low or lacked in purified plasma cells in some patients. MiR 15a, 15b, 16 levels were lower in plasma cells of MM than those of MGUS. On the contrary, Dnmts and Mcl-1 levels were higher in plasma cells of MM than those of MGUS.