Comprehensive gene expression analysis of cancer-infiltrating lymphocytes and peripheral blood cells for elucidation of cancer immunity kinesis

采用小鼠皮下血肿模型，分析局部肿瘤浸润性炎症细胞和外周血细胞的基因表达谱。外周血基因表达谱的特征因注射癌细胞后天数的不同而不同。肿瘤体积最大的小鼠局部肿瘤浸润性炎症细胞和外周血细胞基因表达的共同特征是IL-6信号和对未展开蛋白的反应。在基因表达的个体化过程中，干扰素信号、NK细胞杀伤作用、局部肿瘤浸润性炎症细胞的补体系统、外周血的IL-10抗炎反应、两性霉素信号分别具有各自的特点。提示综合分析局部癌、炎症反应和外周血细胞的基因表达，将揭示局部和全身炎症反应的共性和差异性。

Using murine subcutaneous hapatoma model which shows the proliferating and succeeding extinguishing phases, analysis of gene expression profile for local cancer-infiltrating inflammatory cells as well as peripheral blood cells was performed. The features of gene expression profiles for peripheral blood were distinct depending on the groups divided by the days after cancer cells injection. The common feature of gene expression of local cancer-infiltrating inflammatory cells as well as peripheral blood cells for mice with the tumors at the maximum size levels showed IL-6 signal and response to unfoleded proteins. As for the individually characteristic processes of gene expression, interferon signaling, NK cell cytotoxicity, complement system for local cancer-infiltrating inflammatory cells, and IL-10 anti-inflammatory response, amphoterin signaling for peripheral blood, were featured, respectively. These suggest that comprehensive analysis of gene expression of local cancer inflammatory response and peripheral blood cells will reveal the common as well as the distinguished features of local and systemic inflammatory responses.