Research of endothelial progenitor cell-capture STENT

从外周血单个核细胞中分离出人内皮祖细胞（EPS）标记物阳性细胞，采用实时RT-PCR检测其mRNA水平。进入人单核细胞的KDR阳性细胞很少。内皮集落形成细胞（早期EPS）在培养后第14 ~ 21天鉴定。我们采用荧光显微镜对早期EPC进行免疫分型，并采用非定量RT-PCR对早期EPS进行表征。建立了EPSs的实验鉴定方法。然后，麻醉后的2月龄家猪（体重25±5 kg）在左冠状动脉内植入25个epc捕获支架（n=13）和25个聚合物包被支架（n=13）。在支架置入后14天切除动脉。苏木精-伊红染色进行组织学分析，电镜观察支架支撑表面。在形态计量学分析中，在切片中计算每层血管壁。所有猪均手术成功，所有支架均通畅无血栓形成。电镜显示支架支撑表面内皮化均匀，苏木精-伊红染色显示内膜增厚薄且均匀。

The positive cell of human endothelial progenitor cell (EPS) markers were isolated from peripheral blood mononuclear cell and were analyzed mRNA level by real time RT-PCR. There were few KDR positive cells into the human mononuclear cell. Endothelial colony formation cells (early EPS) colonies were identified on day 14 to 21 after it were cultured. We assessed immunophenotyping of early EPC using fluorescence microscope and characterized the early EPS by non quantitative RT-PCR. We established method of experiment for identification of EPSs. Then, anesthetized domestic swine (weight 25±5 kg, 2 months old) were implanted 25 EPC-capture stents (n=13) and 25 polymer-coated stents (n=13) into left coronary artery. The arteries were harvested at 14 days after the stenting. Histological analysis was performed by hematoxylin-eosin staining and stent strut surface were assessed by electron microscopy. In morphometric analysis, each layer of vascular wall was calculated in the section. Procedural success was achieved in all swine and all stents were patent without thrombosis. Electron microscopy suggest that uniformly endothelialization at the stent strut surface, and hematoxylin-eosin staining showed that neointimal thickining were thin and uniformly.