Development of transcriptional Zn(II) sensor based on protein conformational change

DNA结合蛋白在医学和生命科学领域中受到广泛关注，并被应用于特定基因的靶向和转录调控。在这个项目中，一个锌（II）传感器蛋白，结合到DNA，是通过使用Ant-F（Ant-F）设计的。Ant-F是一种人工合成的蛋白质，它通过与Zn（II）结合而引起结构变化。这项研究将提供一个基本的工具，这可能导致的技术的发展，检测锌（II）的转录活性。

DNA-binding proteins have been attracting attention in the field of medical and life sciences and applied to the targeting and transcriptional control of particular genes. In this project, a Zn(II)-sensor protein, which binds to DNA, is designed by utilizing Antennafinger (Ant-F). Ant-F is an artificial protein, which induce structural change by binding Zn(II). This research will provide a basic tool, which could lead to the development of the technique for the detection of Zn(II) by transcriptional activity.