Fluorescence diagnosis of lung cancer and malignant mesothelioma using orthotopic ijplamtation SCID mice model

将人肺癌细胞株A549、MA44-3和人恶性间皮瘤细胞株MST0211H分别接种于重症联合免疫缺陷病小鼠左肺或左胸腔内。植入后1~3周分别给予5-氨基乙酰丙酸或L-天冬氨酸单氯。这些小鼠在给药几个小时后被处死。打开胸壁，用蓝光(405 Nm)照射。肺肿瘤、转移性淋巴结和胸膜肿瘤在白光下肉眼看不见，但仍可观察到清晰的红色荧光。这一结果提示荧光诊断胸膜内恶性肿瘤的临床应用的可能性。

Human lung cancer cell line A549, Ma44-3 and human malignant mesothelioma cell line MST0211H were injected into left lung or left pleural cavity of severe combined immunodeficiency disease mice. The mice were administered 5-aminolevulinic acid or mono-L-aspartyl chlorine e6 1 to 3 weeks after the implantation. The mice were sacrificed several hours after the administration of these drugs. Their chest walls were opened and illuminated with blue light (405nm). Clear red fluorescence was observed in lung tumors, metastatic lymph nodes, and pleural tumors, even though the tumors were invisible macroscopically under white light. This result suggests possibility of clinical application of fluorescence diagnosis with intrapleural malignant tumors.