The Role and Inhibitory Effect of Myostatin for Muscle Regeneration in Human Urethral Sphincter

尿道横纹肌主要由慢肌纤维组成，具有持续收缩的功能，在排尿机制中起重要作用。本研究以人尿道横纹肌（human urinary rhabdoshincter，HUR）卫星细胞（striated muscle stem cells）为研究对象，探讨骨骼肌成肌负调控因子肌抑素（myostatin，MST）对HUR卫星细胞生长分化的作用机制。MST通过磷酸化smad-2和细胞周期阻滞抑制HUR卫星细胞增殖。相反，FS抑制这些影响。另一方面，目前的研究结果不能表明HUR卫星细胞具有MST自身抑制生长的自分泌作用。

Urethral rhabdoshincter that mainly consist of slow muscle fibers, have an important role of urinary continence mechanism with continuous contraction. Thus, dysfunction of urethral rhabdoshincter caused intractable urinary incontinence.I this research, we studied the mechanism of growth and differentiation in human urethral rhabdoshincter (HUR) satellite cells (striated muscle stem cells) by myostatin (MST), which was a negative regulator of myogenesis of skeletal muscle. MST inhibited the proliferation of HUR satellite cells through phosphorylated smad-2 and cell cycle arrest. On the contrary, FS suppressed these effects. On the other hand, current results could not indicate that HUR satellite cells had an autocrine action of MST for the growth inhibition by itself.