How do bone cell sense mechanical stress?-the role of the actin binding protein-

我们观察了FSS对MC3T3-E1细胞CTGF表达的影响，并探讨了其作用机制。FSS以剂量依赖的方式诱导CTGF表达，最高可达1.5pA，诱导倍数超过5倍。这一作用可被Rho GTP酶抑制剂Y27632抑制。此外，抑制肌动蛋白聚合可阻断FSS诱导的CTGF的激活，而茉莉花内酯和细胞松弛素D强迫F-肌动蛋白的形成可增强CTGF的表达。总之，这些发现表明Rho信号通路在FSS通过诱导CTGF诱导的骨重建中所起的作用。

we evaluated the effect of FSS on ctgf expression and investigated its mechanism in MC3T3-E1 cells. The ctgf expression was induced by FSS in a dose dependent manner up to 1.5 Pa, at which more than 5-fold induction was observed. This effect was inhibited by a Rho GTPase inhibitor, Y27632. Moreover, inhibition of actin polymerization blocked the FSS-induced activation of ctgf, whereas forced F-actin formation by jasplakinolide and cytochalasin D enhanced the ctgf expression in the same cells. Collectively, these findings indicate the role of Rho signaling pathway in bone remodeling provoked by FSS through the induction of CTGF.