Analysis of inhibitory mechanism and antithrombotic effects of a novel anti-platelet function molecule, semaphorin 3A

我们分析了信号蛋白3A抑制血小板功能的机制。除了通过抑制Rac-paxillin通路抑制肌动蛋白聚合外，我们发现semaphorin 3A还抑制pi3k激酶、Rap1和AKT的下游分子。有趣的是，semaphorin 3A抑制凝血酶或惊厥欣诱导的Rap1和AKT的激活，它主要抑制adp诱导的AKT磷酸化。上述结果表明，semaphorin 3A抑制PI3激酶通路，可能对adp诱导的AKT活化具有直接抑制作用。

We analyzed the mechanism of inhibition of platelet function by semaphorin 3A. In addition to inhibition of actin polymerization via suppression of Rac-paxillin pathway, we found that semaphorin 3A inhibits downstream molecules of PI3 kinase, Rap1 and AKT. Interestingly, semaphorin 3A inhibited both thrombin-or convulxin-induced Rap1 and AKT activation, it inhibited mainly ADP-induced AKT phosphorylation. These results indicate that semaphorin 3A inhibits PI3 kinase pathway, and it may have direct inhibitory effects on ADP-induced AKT activation.