The role of glomerular endothelial cell and accelerated regenerative factors in renal injur

我们研究了HUS小鼠肾脏恢复的过程及其与血管内皮生长因子、血管生成素-1的关系。将C57BL/6小鼠随机分为3组。第1组小鼠按30 0μg/kg和2 2 5 ng/kg Stx2剂量腹腔注射脂多糖，第2组小鼠按10 0μg/kg和10 0 ng/kg Stx2小剂量腹腔注射脂多糖。对照组小鼠接种生理盐水。每组5只小鼠于给药后随时间处死取肾进行组织学评价。第1组小鼠血清CRS水平高于第2组，第1组小鼠72~80h全部死亡，第2组小鼠全部存活。两组均可见红细胞碎裂。2组大鼠肾小球系膜细胞增殖、系膜基质表达、CD31阳性表达均高于1组。肾小球血管内皮生长因子和血管生成素-1的表达逐渐增强，72小时至7天达高峰。小剂量内毒素和Stx2诱导的HUS小鼠是急性肾损伤恢复过程中有用的模型，而血管内皮生长因子和血管生成素-1在其恢复过程中起重要作用。

We examined the process of the renal recovery in mice with HUS and the relationship between its renal recovery and VEGF, Angiopoietin-1. C57BL/6 mice were divided into three groups. Group 1 was consisted of mice received an inyraperitoneal injection of dose of LPS at 300 μ g/kg and 225 ng/kg Stx2, Group 2 was consisted of mice received an inyraperitoneal injection of a low dose of LPS at 100 μ g/kg and 100 ng/kg Stx2. Mice in the control group were inoculated with saline. The kidneys were extirpated from 5 mice of each group sacrificed with time after administration for histological evaluation. Serum Crs in Group 1 were higher than those in Group 2 and all mice in Group 1 were died at 72-80 hours and all mice in Group 2 were alive. The fragmented erythrocytes were found in both groups. Endothelial injury and mesangiolysis scores at 24 hours in Group 1 were higher than those in Group 2. In addition, mesangial proliferation and mesangial matrix scores, CD31 positive expression at 72 hours in Group 2 were higher than those in Group 1. Glomerular VEGF and Angiopoietin-1 expressing in Group 2 gradually increased and were peak from 72 hours to 7 days. HUS mice induced by low dose of LPS and Stx2 were useful model for the recovery process of acute renal injury and VEGF and Angiopoietin-1 play important roles of its recovery process.