Development of the device of real-time direct measurement of spinal cord blood flow at the site of compression

我们能够实时观察压迫部位的血流量，并使用一种新的测量血流量的装置获得大鼠脊髓损伤模型的临界压缩力(20g)。我们发现，20分钟的缺血/压迫可导致可逆性的后肢运动功能损害。然而，40分钟的时间会导致不可逆转的瘫痪。其损伤机制包括小胶质细胞增殖和活化所致的炎症反应、神经元和少突胶质细胞的凋亡以及血脊髓屏障的损伤。

We were able to observe real-time blood flow at the site of compression and to obtain the critical compression force (20g) for a rat spinal cord injury model using a novel device to measure blood flow. We found that 20 minutes of ischemia/compression periods induces reversible hindlimb motor function damage. However, 40 minutes of that produces irreversible paralysis. The mechanisms of the damage include inflammation induced by proliferated and activated microglia, apoptosis in neuron and oligodendrocyte and blood-spinal cord barrier damage.