Type of bone invasion in human or al squamous cell carcinoma and its mechanisms ; application for molecular diagnosis

我们用免疫组织化学方法观察了与破骨细胞分化相关的各种细胞因子在人下牙龈鳞状细胞癌中的表达。虽然PTHrP有少量表达，但RANKL和OPG在癌细胞中几乎没有过表达。MMP9和Periostin在高侵袭性细胞系(HSC4)和低侵袭性细胞系(HSC3)中高表达。小剂量双膦酸盐可诱导HSC4细胞发生凋亡。此外，钙还可促进细胞的凋亡诱导。

We immunohistochemically observed expression of various kinds of cytokines related to osteoclastic differentiation in human squamous cell carcioma of the lower gum. Over-expression of RANKL or OPG could be barely seen in cancer cells, although PTHrP was slightly expressed. The relationship between type of bone invasion and expression of them has failed to clarify.Using oral squamous cancer cell lines, over-expression of MMP9 or periostin was strongly observed in high invasive cell line (HSC4) compared with low invasive cell line (HSC3). Apoptosis was induced in HSC4 cells with low dose bisphosphonate. Moreover, induction of apoptosis in cell line was enhanced by addition to Ca.